|
| Status |
Public on Nov 04, 2014 |
| Title |
TMK2 |
| Sample type |
RNA |
| |
|
| Source name |
Tympanic membrane keratinocyte
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: keratinocyte
tissue: Tympanic membrane
|
| Treatment protocol |
No treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from samples of cultivated TMK and EK cells was extracted using the miRNeasy mini kit from Qiagen
|
| Label |
biotin
|
| Label protocol |
Total RNA (150 ng) from each sample was prepared using the Ambion WT Expression Kit (Ambion Inc., Austin, Texas, USA) according to manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Fragmented and labeled sense strand DNA was hybridized to the GeneChip Human Gene 2.0 ST Array (Affymetrix, Santa Clara, CA). The arrays were washed and stained using FS-450 fluidics station (Affymetrix).
|
| Scan protocol |
Fluorescent images were scanned by Hewlett Packard Gene Array Scanner 3000 7G (Hewlett Packard, Palo Alto, CA).The scanned images were then analyzed using Affymetrix GeneChip Command Console
|
| Description |
Primary cells from surgical patients
|
| Data processing |
The CEL files were imported into Partek Genomics Suite software (Partek, Inc., St. Louis, MO). Robust microarray analysis (RMA) was applied for normalization. Exon array data were filtered to include only those probe sets derived from the core meta-probe list.
|
| |
|
| Submission date |
Nov 03, 2014 |
| Last update date |
Nov 04, 2014 |
| Contact name |
Peder Aabel |
| E-mail(s) |
peder.aabel@medisin.uio.no
|
| Organization name |
Akershus University Hospital
|
| Department |
ENT
|
| Street address |
Sykehusvn. 27
|
| City |
Lorenskog |
| ZIP/Postal code |
1478 |
| Country |
Norway |
| |
|
| Platform ID |
GPL16686 |
| Series (2) |
| GSE62939 |
Expression data of human tympanic membrane cells and epidermal keratinocytes [mRNA] |
| GSE76674 |
Expression data of human tympanic membrane cells and epidermal keratinocytes |
|
