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Sample GSM1544085 Query DataSets for GSM1544085
Status Public on Dec 01, 2018
Title 16HBE-pQCXIP/GSK1120212-n2
Sample type RNA
 
Source name 16HBE, pQCXIP infected (stable), 500nM GSK1120212 treated (4 days)
Organism Homo sapiens
Characteristics cell line: 16HBE
cell type: bronchial epithelial immortalised cell line
Treatment protocol pQCXIP cells were treated with DMSO (control), 500nM GSK1120212 (MEK inhibitor) or 1uM SCH772984 (ERK inhibitor) for 4 days
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions using Qiagen RNA kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Illumina protocol from total RNA.
 
Hybridization protocol Following fragmentation, cRNA were hybridized for Illumina GX human HT12 Array. Slides were washed and stained in the Illumina instrument.
Scan protocol Slides were scanned using Illumina iScanner
Description Expression profile of Ras, MEK and ERK inhibited 16HBE bronchial epithelial cells
GSK_2
Data processing The data were analyzed with GenomeStudio using Illumina default analysis settings. No normalization and background correction are performed first, then quantile normalization and background correction are done.
 
Submission date Nov 12, 2014
Last update date Dec 01, 2018
Contact name Jeffrey Zhao
Organization name Memorial Sloan Kettering Cancer Center
Department Genomics Core
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL10558
Series (1)
GSE63229 Expression profile of Ras, MEK and ERK inhibited 16HBE bronchial epithelial cells

Data table header descriptions
ID_REF
VALUE Normalized signal
GSK_2.Avg_NBEADS
GSK_2.BEAD_STDERR
GSK_2.Detection Pval

Data table
ID_REF VALUE GSK_2.Avg_NBEADS GSK_2.BEAD_STDERR GSK_2.Detection Pval
ILMN_1762337 -1.385098 26 2.436871 0.6220779
ILMN_2055271 9.373057 17 4.165562 0.02597403
ILMN_1736007 3.394344 25 2.446389 0.174026
ILMN_2383229 -6.286214 28 1.924098 0.9571428
ILMN_1806310 0.8391875 30 2.01318 0.3779221
ILMN_1779670 1.819256 28 2.627137 0.2831169
ILMN_1653355 -0.5068144 21 2.600681 0.5181818
ILMN_1717783 1.128123 27 2.485056 0.3493507
ILMN_1705025 7.804499 18 4.078616 0.04025974
ILMN_1814316 -0.6587119 15 2.955964 0.5350649
ILMN_2359168 -0.01630032 28 2.654443 0.4584416
ILMN_1731507 -4.688107 19 1.96867 0.8909091
ILMN_1787689 -3.744857 19 2.7581 0.8181818
ILMN_3241953 22.30674 19 4.005336 0.001298701
ILMN_1745607 3.615129 19 3.113385 0.1571429
ILMN_2136495 -3.145081 29 1.719115 0.7805195
ILMN_1668111 -7.418744 21 2.103533 0.9818182
ILMN_2295559 1.253158 16 3.699847 0.3337662
ILMN_1735045 103.1808 22 7.978165 0
ILMN_1680754 10.15203 15 4.736875 0.01948052

Total number of rows: 47317

Table truncated, full table size 1948 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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