|
Status |
Public on Dec 01, 2018 |
Title |
16HBE-pQCXIP/GSK1120212-n3 |
Sample type |
RNA |
|
|
Source name |
16HBE, pQCXIP infected (stable), 500nM GSK1120212 treated (4 days)
|
Organism |
Homo sapiens |
Characteristics |
cell line: 16HBE cell type: bronchial epithelial immortalised cell line
|
Treatment protocol |
pQCXIP cells were treated with DMSO (control), 500nM GSK1120212 (MEK inhibitor) or 1uM SCH772984 (ERK inhibitor) for 4 days
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions using Qiagen RNA kit.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Illumina protocol from total RNA.
|
|
|
Hybridization protocol |
Following fragmentation, cRNA were hybridized for Illumina GX human HT12 Array. Slides were washed and stained in the Illumina instrument.
|
Scan protocol |
Slides were scanned using Illumina iScanner
|
Description |
Expression profile of Ras, MEK and ERK inhibited 16HBE bronchial epithelial cells GSK_3
|
Data processing |
The data were analyzed with GenomeStudio using Illumina default analysis settings. No normalization and background correction are performed first, then quantile normalization and background correction are done.
|
|
|
Submission date |
Nov 12, 2014 |
Last update date |
Dec 01, 2018 |
Contact name |
Jeffrey Zhao |
Organization name |
Memorial Sloan Kettering Cancer Center
|
Department |
Genomics Core
|
Street address |
1275 York Avenue
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE63229 |
Expression profile of Ras, MEK and ERK inhibited 16HBE bronchial epithelial cells |
|