NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1544086 Query DataSets for GSM1544086
Status Public on Dec 01, 2018
Title 16HBE-pQCXIP/GSK1120212-n3
Sample type RNA
 
Source name 16HBE, pQCXIP infected (stable), 500nM GSK1120212 treated (4 days)
Organism Homo sapiens
Characteristics cell line: 16HBE
cell type: bronchial epithelial immortalised cell line
Treatment protocol pQCXIP cells were treated with DMSO (control), 500nM GSK1120212 (MEK inhibitor) or 1uM SCH772984 (ERK inhibitor) for 4 days
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions using Qiagen RNA kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Illumina protocol from total RNA.
 
Hybridization protocol Following fragmentation, cRNA were hybridized for Illumina GX human HT12 Array. Slides were washed and stained in the Illumina instrument.
Scan protocol Slides were scanned using Illumina iScanner
Description Expression profile of Ras, MEK and ERK inhibited 16HBE bronchial epithelial cells
GSK_3
Data processing The data were analyzed with GenomeStudio using Illumina default analysis settings. No normalization and background correction are performed first, then quantile normalization and background correction are done.
 
Submission date Nov 12, 2014
Last update date Dec 01, 2018
Contact name Jeffrey Zhao
Organization name Memorial Sloan Kettering Cancer Center
Department Genomics Core
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL10558
Series (1)
GSE63229 Expression profile of Ras, MEK and ERK inhibited 16HBE bronchial epithelial cells

Data table header descriptions
ID_REF
VALUE Normalized signal
GSK_3.Avg_NBEADS
GSK_3.BEAD_STDERR
GSK_3.Detection Pval

Data table
ID_REF VALUE GSK_3.Avg_NBEADS GSK_3.BEAD_STDERR GSK_3.Detection Pval
ILMN_1762337 -4.144672 20 3.398457 0.8415585
ILMN_2055271 3.489523 17 3.150019 0.1753247
ILMN_1736007 -6.687049 18 2.038256 0.9675325
ILMN_2383229 1.244039 25 2.080602 0.3441558
ILMN_1806310 -3.244796 17 2.054554 0.774026
ILMN_1779670 5.965349 22 3.437847 0.07272727
ILMN_1653355 -1.642267 20 2.629937 0.6337662
ILMN_1717783 -4.944261 19 1.751729 0.8974026
ILMN_1705025 1.674413 26 2.023777 0.312987
ILMN_1814316 4.93791 25 3.083267 0.1077922
ILMN_2359168 -2.080691 25 1.668598 0.6818182
ILMN_1731507 -0.7409218 23 2.493265 0.5467532
ILMN_1787689 -0.08621927 19 2.462938 0.4701299
ILMN_3241953 34.50325 14 5.00478 0
ILMN_1745607 2.35955 26 2.216806 0.2506494
ILMN_2136495 11.8866 25 3.625812 0.01038961
ILMN_1668111 -2.587788 24 2.759597 0.7220779
ILMN_2295559 4.278775 18 4.064275 0.1311688
ILMN_1735045 94.41036 26 6.934251 0
ILMN_1680754 3.135535 20 4.27883 0.1948052

Total number of rows: 47317

Table truncated, full table size 1946 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.