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Sample GSM1544091 Query DataSets for GSM1544091
Status Public on Dec 01, 2018
Title 16HBE-pQCXIP.DN Ras N17-n2
Sample type RNA
 
Source name 16HBE, pQCXIP-DN Ras infected (stable)
Organism Homo sapiens
Characteristics cell line: 16HBE
cell type: bronchial epithelial immortalised cell line
Treatment protocol pQCXIP cells were treated with DMSO (control), 500nM GSK1120212 (MEK inhibitor) or 1uM SCH772984 (ERK inhibitor) for 4 days
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions using Qiagen RNA kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Illumina protocol from total RNA.
 
Hybridization protocol Following fragmentation, cRNA were hybridized for Illumina GX human HT12 Array. Slides were washed and stained in the Illumina instrument.
Scan protocol Slides were scanned using Illumina iScanner
Description Expression profile of Ras, MEK and ERK inhibited 16HBE bronchial epithelial cells
DN_Ras_2
Data processing The data were analyzed with GenomeStudio using Illumina default analysis settings. No normalization and background correction are performed first, then quantile normalization and background correction are done.
 
Submission date Nov 12, 2014
Last update date Dec 01, 2018
Contact name Jeffrey Zhao
Organization name Memorial Sloan Kettering Cancer Center
Department Genomics Core
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL10558
Series (1)
GSE63229 Expression profile of Ras, MEK and ERK inhibited 16HBE bronchial epithelial cells

Data table header descriptions
ID_REF
VALUE Normalized signal
DN_Ras_2.Avg_NBEADS
DN_Ras_2.BEAD_STDERR
DN_Ras_2.Detection Pval

Data table
ID_REF VALUE DN_Ras_2.Avg_NBEADS DN_Ras_2.BEAD_STDERR DN_Ras_2.Detection Pval
ILMN_1762337 3.715687 26 2.342203 0.161039
ILMN_2055271 9.862968 21 2.617613 0.01948052
ILMN_1736007 -3.174123 25 2.498817 0.7844156
ILMN_2383229 7.758378 27 3.429639 0.04155844
ILMN_1806310 1.932391 20 1.850398 0.2493507
ILMN_1779670 -0.9662439 22 2.648466 0.5337662
ILMN_1653355 2.096348 19 3.356009 0.2402597
ILMN_1717783 0.005643726 23 2.28909 0.4350649
ILMN_1705025 -3.617335 17 2.383288 0.8207792
ILMN_1814316 -6.804625 27 2.439948 0.9675325
ILMN_2359168 -5.629964 17 3.042906 0.9311689
ILMN_1731507 1.740965 21 3.441362 0.2649351
ILMN_1787689 -2.406544 20 2.260306 0.7142857
ILMN_3241953 37.25952 26 3.571845 0
ILMN_1745607 -0.9967875 27 2.45008 0.5402597
ILMN_2136495 3.08992 24 2.656871 0.1922078
ILMN_1668111 -0.1178329 14 3.604021 0.4558442
ILMN_2295559 0.3328945 18 2.13676 0.3974026
ILMN_1735045 72.65466 29 4.558722 0
ILMN_1680754 5.525963 20 2.649603 0.0961039

Total number of rows: 47317

Table truncated, full table size 1943 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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