|
Status |
Public on Jun 12, 2017 |
Title |
Muscle_HFD_narciclasine |
Sample type |
SRA |
|
|
Source name |
Muscle
|
Organism |
Mus musculus |
Characteristics |
treatment: narciclasine strain: C57BL/6J tissue: Muscle age: 17 weeks
|
Treatment protocol |
Drug treatment started from 10 weeks until 17 weeks.
|
Growth protocol |
Male C57BL/6J mice were fed on either a normal chow diet (NCD) or a high fat diet (HFD) from the age of 8 weeks to 17 weeks.
|
Extracted molecule |
total RNA |
Extraction protocol |
Fresh mouse tissues were isolated and snap frozen in liquid nitrogen. Total RNA was prepared using TRIzol reagent and quantified by NanoDrop-1000. Quality control was performed using Agilent 2100 bioanalyzer. 1 ug total RNA was used for sequencing library construction. RNA sequencing libraries were prepared according to the standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
All RNA-seq data sets were aligned to the mouse genome using TopHat The mapped reads were further quantified as fragments per kilobase of transcript per million mapped reads (FPKM) based on the RefSeq database using the Cufflinks program Genome_build: mm9 Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each sample
|
|
|
Submission date |
Nov 13, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Wei Xie |
E-mail(s) |
xiewei121@tsinghua.edu.cn
|
Organization name |
Tsinghua University
|
Street address |
zhongguancun beidajie
|
City |
Beijing |
ZIP/Postal code |
100086 |
Country |
China |
|
|
Platform ID |
GPL13112 |
Series (1) |
GSE63268 |
Effects of narciclasine treatment on major metabolic organs of C57BL/6 mice |
|
Relations |
BioSample |
SAMN03178012 |
SRA |
SRX758987 |