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Sample GSM1544797 Query DataSets for GSM1544797
Status Public on Jun 12, 2017
Title Muscle_HFD_narciclasine
Sample type SRA
 
Source name Muscle
Organism Mus musculus
Characteristics treatment: narciclasine
strain: C57BL/6J
tissue: Muscle
age: 17 weeks
Treatment protocol Drug treatment started from 10 weeks until 17 weeks.
Growth protocol Male C57BL/6J mice were fed on either a normal chow diet (NCD) or a high fat diet (HFD) from the age of 8 weeks to 17 weeks.
Extracted molecule total RNA
Extraction protocol Fresh mouse tissues were isolated and snap frozen in liquid nitrogen. Total RNA was prepared using TRIzol reagent and quantified by NanoDrop-1000. Quality control was performed using Agilent 2100 bioanalyzer. 1 ug total RNA was used for sequencing library construction.
RNA sequencing libraries were prepared according to the standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing All RNA-seq data sets were aligned to the mouse genome using TopHat
The mapped reads were further quantified as fragments per kilobase of transcript per million mapped reads (FPKM) based on the RefSeq database using the Cufflinks program
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each sample
 
Submission date Nov 13, 2014
Last update date May 15, 2019
Contact name Wei Xie
E-mail(s) xiewei121@tsinghua.edu.cn
Organization name Tsinghua University
Street address zhongguancun beidajie
City Beijing
ZIP/Postal code 100086
Country China
 
Platform ID GPL13112
Series (1)
GSE63268 Effects of narciclasine treatment on major metabolic organs of C57BL/6 mice
Relations
BioSample SAMN03178012
SRA SRX758987

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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