|
| Status |
Public on May 15, 2015 |
| Title |
rat_non-basal cells_rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
epididymal non-basal cells
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
age: 48 days
tissue: epididymis
type of cells: non-basal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
extraction of total RNA was performed using Qiagen's kit according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard affymetrix protocol from 100ng of total RNA. After purification of cRNA, 15ug were used to generate cDNA.
|
| |
|
| Hybridization protocol |
Hybridization was done according to Affymetrix protocol using GeneChip WT Plus Reagent kit. 5,5uf of cDNA were hybridized at 45 during 16 hrs. Wash/stain step was performed with GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChipScanner 3000 7G autoloader with an excitation wavelength of 488nm.
|
| Description |
DCR007
|
| Data processing |
Data were RMA-normalized with Expression console and further analyzed with GeneSpring 12.6 software.
affymetrix-algorithm-name = rma-gene-full
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.3.1.187
|
| |
|
| Submission date |
Nov 14, 2014 |
| Last update date |
May 15, 2015 |
| Contact name |
Daniel Cyr |
| E-mail(s) |
daniel.cyr@iaf.inrs.ca
|
| Organization name |
INRS-IAF
|
| Street address |
531 boulevard des Prairies
|
| City |
laval |
| State/province |
Québec |
| ZIP/Postal code |
H7V 1B7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE63305 |
Gene expression profiling of basal cells in rat epididymis |
|
