Triazol (Invitrogen, Carlsbad, CA) regent followed by RNeasy (Qiagen, Valencia, CA) lipid mini kit cleanup
Label
biotin
Label protocol
Affymetrix recomended labelling protocol was used.
Hybridization protocol
Affymetrix recomended hybridization protocol was used.
Scan protocol
U133A GeneChips were scanned using a Affymetrix GeneChip Scanner 7G according to the manufactureres instructions.
Description
None
Data processing
Raw values obtained from Affymetrix Cel files were preprocessed for RMA in GeneSpring GX (version 7.3.1) followed by per-gene and per-chip median polishing.
