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Sample GSM1547744 Query DataSets for GSM1547744
Status Public on Dec 31, 2016
Title IBS_5
Sample type RNA
 
Source name PBMCs_IBS
Organism Homo sapiens
Characteristics disease status: IBS
tissue: blood
cell type: peripheral blood mononuclear cells
Extracted molecule total RNA
Extraction protocol PBMCs were extracted from blood using standard protocols. RNA was isolated using the Qiagen RNeasy mini Kit (cat 74104). Column based.
Label biotin
Label protocol After Purification, single-stranded cDNA was fragmented and labeled using the GeneChip WT Terminal Labeling kit from Affymetrix (900671)
 
Hybridization protocol The fragmented single-stranded cDNA was combined with hybridization controls from Affymetrix and hybridized in the HTA 2.0 array overnight for 16-18 hours in the Affymetrix Genechip Hybridization Oven 645
Scan protocol Arrays were stained and washed in the Affymetrix GeneChip Fluidics Station 450. Mircoarrays were scanned using the Affymetrix GeneChip Scanner 3000.
Data processing Raw data was extracted and preprocessed using Expression Console from Affymetrix. CEL files were generated by the scanner.
 
Submission date Nov 17, 2014
Last update date Dec 31, 2016
Contact name Chang Lin
Organization name University of California, Los Angeles
Department Medicine
Lab Center for Neurobiology of Stress
Street address 10833 Le Conte Avenue, CHS 47-122
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL17586
Series (1)
GSE63379 Genome-wide Expression Profiling in Irritable Bowel Syndrome

Data table header descriptions
ID_REF
VALUE Robust Multi-Array (RMA) signal

Data table
ID_REF VALUE
TC01000001.hg.1 7.4
TC01000002.hg.1 6.14
TC01000003.hg.1 4.27
TC01000004.hg.1 4.74
TC01000005.hg.1 9.58
TC01000006.hg.1 12.02
TC01000007.hg.1 12.25
TC01000008.hg.1 7
TC01000009.hg.1 2.9
TC01000010.hg.1 5.23
TC01000011.hg.1 7.36
TC01000012.hg.1 6.73
TC01000013.hg.1 9.8
TC01000014.hg.1 4.87
TC01000015.hg.1 6.93
TC01000016.hg.1 7.48
TC01000017.hg.1 6.87
TC01000018.hg.1 6.94
TC01000019.hg.1 7.46
TC01000020.hg.1 7.79

Total number of rows: 70523

Table truncated, full table size 1449 Kbytes.




Supplementary file Size Download File type/resource
GSM1547744_A5169.CEL.gz 24.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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