|
Status |
Public on Feb 15, 2007 |
Title |
Root_tZ-treatment_30min_replicate 3 |
Sample type |
RNA |
|
|
Source name |
Oryza sativa root, tZ-treated, 30 min
|
Organism |
Oryza sativa |
Characteristics |
Strain: Oryza sativa L. cv Nipponbare, Tissue: root, Age: two weeks after germination
|
Treatment protocol |
Whole roots were excised from the two-week-old seedlings and immediately dipped in distilled water containing either 5 µM trans-zeatin (tZ) and 0.1% dimethylsulfoxide (DMSO). The excised organ was incubated at 30°C for 30 or 120 min, harvested and stored at -80°C until the RNA was extracted.
|
Growth protocol |
Rice (Oryza sativa L. cv Nipponbare) seeds were sterilized with 70 % ethanol for 1 min and then with 2 % (w/v) sodium hypochlorite for 15 min, soaked in distilled water at 30°C for 1 d and 30 germinated seeds were transferred to a nylon net floating on tap water that had been adjusted to pH 5.5. Seedlings were grown for 2-weeks in an environment-controlled greenhouse with a photoperiod of 12 h light (30°C)/12 h dark (25°C).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was prepared with the RNeasy plant Mini Kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.
|
|
|
Hybridization protocol |
standard Affymetrix procedures
|
Scan protocol |
standard Affymetrix procedures
|
Description |
Publication: Naoya Hirose, Nobue Makita, Mikiko Kojima, Tomoe Kamada-Nobusada and Hitoshi Sakakibara (2007) Over-expression of a Type-A Response Regulator Alters Rice Morphology and Cytokinin Metabolism. Plant Cell Physiology, 48: in press.
|
Data processing |
GCOS1.2
|
|
|
Submission date |
Jan 11, 2007 |
Last update date |
Feb 15, 2007 |
Contact name |
Hitoshi Sakakibara |
E-mail(s) |
sakaki@riken.jp
|
Phone |
81-45-503-9576
|
Fax |
81-45-503-9609
|
Organization name |
RIKEN
|
Department |
Plant Science Center
|
Lab |
Biodynamics Research Team
|
Street address |
1-7-22 Suehiro, Tsurumi
|
City |
Yokohama |
ZIP/Postal code |
230-0045 |
Country |
Japan |
|
|
Platform ID |
GPL2025 |
Series (2) |
GSE6719 |
Cytokinin responsive genes in rice |
GSE6737 |
Over-expression of a Type-A Response Regulator Alters Rice Morphology and Cytokinin Metabolism |
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