|
| Status |
Public on Feb 15, 2007 |
| Title |
Root_tZ-treatment_120min_replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Oryza sativa root, tZ-treated, 120 min
|
| Organism |
Oryza sativa |
| Characteristics |
Strain: Oryza sativa L. cv Nipponbare, Tissue: root, Age: two weeks after germination
|
| Treatment protocol |
Whole roots were excised from the two-week-old seedlings and immediately dipped in distilled water containing either 5 µM trans-zeatin (tZ) and 0.1% dimethylsulfoxide (DMSO). The excised organ was incubated at 30°C for 120 min, harvested and stored at -80°C until the RNA was extracted.
|
| Growth protocol |
Rice (Oryza sativa L. cv Nipponbare) seeds were sterilized with 70 % ethanol for 1 min and then with 2 % (w/v) sodium hypochlorite for 15 min, soaked in distilled water at 30°C for 1 d and 30 germinated seeds were transferred to a nylon net floating on tap water that had been adjusted to pH 5.5. Seedlings were grown for 2-weeks in an environment-controlled greenhouse with a photoperiod of 12 h light (30°C)/12 h dark (25°C).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared with the RNeasy plant Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Publication: Naoya Hirose, Nobue Makita, Mikiko Kojima, Tomoe Kamada-Nobusada and Hitoshi Sakakibara (2007) Over-expression of a Type-A Response Regulator Alters Rice Morphology and Cytokinin Metabolism. Plant Cell Physiology, 48: in press.
|
| Data processing |
GCOS1.2
|
| |
|
| Submission date |
Jan 11, 2007 |
| Last update date |
Feb 15, 2007 |
| Contact name |
Hitoshi Sakakibara |
| E-mail(s) |
sakaki@riken.jp
|
| Phone |
81-45-503-9576
|
| Fax |
81-45-503-9609
|
| Organization name |
RIKEN
|
| Department |
Plant Science Center
|
| Lab |
Biodynamics Research Team
|
| Street address |
1-7-22 Suehiro, Tsurumi
|
| City |
Yokohama |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2025 |
| Series (2) |
| GSE6719 |
Cytokinin responsive genes in rice |
| GSE6737 |
Over-expression of a Type-A Response Regulator Alters Rice Morphology and Cytokinin Metabolism |
|
