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Status |
Public on Nov 21, 2014 |
Title |
Kidney_UPEC132_infected_4h_rep1 |
Sample type |
RNA |
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Source name |
Mouse kidney of UPEC132 group at 4h post-infection
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Organism |
Mus musculus |
Characteristics |
time: 4 h infection: E. coli UPEC132 genetic background: BALB/c tissue: kidney gender: female age: 8 weeks weight: 16-18 g
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the the RNeasy Plus kit (Qiagen Inc., Valencia, CA, USA) and the RNAprotect reagent (Qiagen) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen). RNA quality and concentration was determined by analysis with agarose gel electrophoresis and spectrophotometer at CapitalBio Corporation (Beijing, China).
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Label |
Cy3
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Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol
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Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol.
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Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol.
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Description |
This sample is of kidney from BALB/c mouse at 4 h post-infection by UPEC132. It is the first of two biological replicates used in this experiment, each from separate mouse.
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Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
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Submission date |
Nov 20, 2014 |
Last update date |
Nov 21, 2014 |
Contact name |
Dongjing Yang |
E-mail(s) |
yangdongjing@cdctj.gov.cn
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Organization name |
Tianjin Centers for Disease Control and Prevention
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Department |
Tianjin Institute for Preventive Medicine
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Street address |
6, Huayue Road
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City |
Tianjin |
ZIP/Postal code |
300171 |
Country |
China |
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Platform ID |
GPL15887 |
Series (1) |
GSE63502 |
Functional analysis of the uropathogenic Escherichia coli R049 gene |
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