|
| Status |
Public on Jul 31, 2015 |
| Title |
PR20_2 [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
hormone-refractory prostate cancer cell lines, 20 nM Paclitaxel resistant PC3
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
tissue: prostate cancer
cell line background: PC3
cell line: PC3PR20
cell type: 20 nM Paclitaxel resistant PC3 cells
paclitaxel resistance: 20 nM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA containing miRNA was extracted using the miRNeasy Mini Kit (Qiagen, Hilden, Germany).
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled miRNA was prepared from 0.1 ug RNA using the Agilent miRNA Complete Labeling and Hybridization Kit (Agilent) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
The labeled miRNAs were hybridized to microarrays in a rotating hybridization oven at 10 rpm for 17 h at 55 °C.
|
| Scan protocol |
After hybridization, the microarrays were washed according to the manufacturer’s protocol and scanned on an Agilent DNA Microarray Scanner G2505C with the Scan Control software (Agilent Technologies).
|
| Description |
r2_2
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7 (Agilent) using default parameters (protocol miRNA_107_Sep09 and Grid: 019118_D_F_20100604) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Dec 09, 2014 |
| Last update date |
Jul 31, 2015 |
| Contact name |
Toshio Kojima |
| Organization name |
Toyohashi University of Technology
|
| Department |
Health Care Center
|
| Street address |
1-1 Hibarigaoka Tenpaku-cho
|
| City |
Toyohashi |
| ZIP/Postal code |
441-8580 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7731 |
| Series (2) |
| GSE64013 |
miR-130a activates apoptotic signaling through activation of caspase-8 in taxane-resistant prostate cancer cells [miRNA] |
| GSE64014 |
miR-130a activates apoptotic signaling through activation of caspase-8 in taxane-resistant prostate cancer cells. |
|
