|
| Status |
Public on Jan 07, 2015 |
| Title |
HCT116 0.05% DMSO 12h_replicates 1 and 2 |
| Sample type |
RNA |
| |
|
| Source name |
HCT116
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
cell type: colorectal cancer cell type
treatment: DMSO control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent (Life Technologies) according to the manufacturers protocol. To confirm RNA samples were not degraded, RNA quality was checked with the Experion StdSens kit (Bio-Rad) prior to amplification and labeling.
|
| Label |
biotin
|
| Label protocol |
The Illumina TotalPrep RNA Amplification Kit was used according to the manufacturer’s instructions to amplify and label RNA samples for Illumina array hybridization
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicates 1 and 2
|
| Data processing |
The data was analyzed and exported from Illumina’s GenomeStudio software using quantile normalization without background subtraction
|
| |
|
| Submission date |
Dec 10, 2014 |
| Last update date |
Jan 07, 2015 |
| Contact name |
Seth Frietze |
| E-mail(s) |
seth.frietze@med.uvm.edu
|
| Phone |
8026560569
|
| Organization name |
University of Vermont
|
| Department |
BHSC
|
| Street address |
106 Carrigan Drive
|
| City |
Burlington |
| State/province |
VT |
| ZIP/Postal code |
05405 |
| Country |
USA |
| |
|
| Platform ID |
GPL10904 |
| Series (2) |
| GSE64038 |
Altering cancer transcriptomes using epigenomic inhibitors [Illumina beadchip] |
| GSE64039 |
Altering cancer transcriptomes using epigenomic inhibitors |
|
