|
| Status |
Public on Apr 24, 2016 |
| Title |
Emb_MO-Tbx5a_48hpf_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Embryos, MO-Tbx5a, 48hpf, replicate2
|
| Organism |
Danio rerio |
| Characteristics |
tissue: embryo
strain: AB
treatment: Mo-Tbx5a injection
developmental stage: 48hpf
|
| Treatment protocol |
1-cell stage zebrafish embryos were microinjected with 1,5 ng of morpholino against dre-Tbx5a (MO-Tbx5a) or with 1,5 ng of control morpholino
|
| Growth protocol |
Zebrafish was raised and maintained under standard laboratory conditions (Westerfileld M zebrafish book) in Zebrafish Housing Systems (Tecniplast, Varese, Italy).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using miRNeasy Mini kit (Qiagen) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng RNA using LowInput QuickAmp Labeling Kit One-Color (Agilent Technologies) according to the manufacturer's instructions, followed by RNAeasy column purification.
|
| |
|
| Hybridization protocol |
Microarray hybridizations were carried out in Agilent’s SureHyb Hybridization Chambers containing 1650 ng of Cyanine 3-labelled cRNA per hybridization. The hybridization reactions were performed at 65°C for 17 hours using Agilent’s Gene Expression Hybridization Kit with the 4x44K whole Danio rerio genome chip (grid ID 026437, G2519F chip). After hybridization, slides were washed with Agilent Gene Expression Wash Buffer 1, Gene Expression Wash Buffer 2 and Acetonitrile for one minute at room temperature.
|
| Scan protocol |
Chips were scanned on an Agilent G2505C scanner.
|
| Description |
Gene expression after Mo-Tbx5a injection
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.7) and the GE1_107_Sep09 extraction protocol.
|
| |
|
| Submission date |
Dec 22, 2014 |
| Last update date |
Apr 24, 2016 |
| Contact name |
Romina D'Aurizio |
| E-mail(s) |
romina.daurizio@gmail.com
|
| Organization name |
CNR
|
| Department |
IIT
|
| Street address |
via Moruzzi 1
|
| City |
Pisa |
| State/province |
PI |
| ZIP/Postal code |
56127 |
| Country |
Italy |
| |
|
| Platform ID |
GPL14664 |
| Series (2) |
| GSE64426 |
Discovering miRNA regulatory networks in Holt-Oram Syndrome using a Zebrafish model [array] |
| GSE64466 |
Discovering miRNA regulatory networks in Holt-Oram Syndrome using a Zebrafish model |
|
