|
Status |
Public on Dec 29, 2014 |
Title |
68_D7 |
Sample type |
SRA |
|
|
Source name |
PBMC
|
Organism |
Macaca fascicularis |
Characteristics |
immunization: MARVgp timepoint (days): day 7 outcome: succumb cell type: PBMC
|
Growth protocol |
PBMCs
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen miRNeasy Isolation Kit from Trizol homogenates Illumina mRNA
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina Genome Analyzer IIx |
|
|
Data processing |
Reads were basecalled using SCS version 2.9 software and processed using version CASAVA 1.8.2 zipped using gzip For mRNAseq: Reads were mapped against the Macaca mulatta using Illumina's igenomes source Ensembl, Build: Mmul_1. The transcriptome was mapped using Bowtie/Tophat . HTSeq was used to generate gene counts Genome_build: Mmul_1 Supplementary_files_format_and_content: tab-delimited text files include: 1 scaled gene counts for each sample of mRNAseq
|
|
|
Submission date |
Dec 29, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Michael Katze |
E-mail(s) |
data@viromics.washington.edu
|
Organization name |
University of Washington
|
Department |
Microbiology
|
Lab |
Michael G. Katze, Ph.D
|
Street address |
Rosen Building 960 Republican St.
|
City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98109-4325 |
Country |
USA |
|
|
Platform ID |
GPL17720 |
Series (1) |
GSE64538 |
Next generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with VSVΔG/ZEBOVgp |
|
Relations |
BioSample |
SAMN03273503 |
SRA |
SRX824800 |