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Sample GSM1577830 Query DataSets for GSM1577830
Status Public on Jan 08, 2015
Title Interscapular brown adipose tissue in mice fed Normal diet 2 weeks rep3
Sample type RNA
 
Source name Interscapular brown adipose_Normal diet_2 wks
Organism Mus musculus
Characteristics strain: C57BL/6J
age: 7 weeks
diet: Normal diet
time point: 2 weeks
tissue: Interscapular brown adipose tissue
Treatment protocol C57BL/6J mice were fed a high-fat diet (HFD) or normal diet (ND) and sacrificed at 5 time-points (2, 4, 8, 20 and 24 weeks) over 24 weeks.
Growth protocol All the mice were individually housed under a constant temperature (24°C) and 12-hour light/dark cycle, fed the AIN-76 semi-purified diet or AIN-76 semi-purified diet with 39.2 kcal% fat (American Institute of Nutrition, 1977) consisting of 20% fat and 1% cholesterol.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 3
B2N3
Data processing Raw data were extracted using the software provided by the manufacturer (Illumina BeadStudio v3.1.3 (Gene Expression Module v3.3.8). The data were normalised using quantile normalisation with beadarray in R
 
Submission date Jan 07, 2015
Last update date Jan 08, 2015
Contact name Sanggyu Lee
E-mail(s) slee@knu.ac.kr
Organization name Kyungpook National University
Street address 1370 Sankyuk-dong, Bukgu
City Daegu
ZIP/Postal code 702-701
Country South Korea
 
Platform ID GPL6887
Series (1)
GSE64718 Gene expression profiles reveal the effect of a high-fat diet in brown fat development

Data table header descriptions
ID_REF
VALUE Quantile normalized (log2)

Data table
ID_REF VALUE
ILMN_2417611 6.794186204
ILMN_2762289 7.130373354
ILMN_2896528 13.23317633
ILMN_2721178 11.74767905
ILMN_2458837 6.826481793
ILMN_3033922 12.04096793
ILMN_3092673 13.70256843
ILMN_1230777 12.71629088
ILMN_1246069 14.38776041
ILMN_1232042 6.852238331
ILMN_1243193 10.67615466
ILMN_2524361 6.895406278
ILMN_1242440 7.027891953
ILMN_1233188 7.738164073
ILMN_2543688 10.24792014
ILMN_1259789 7.966164693
ILMN_2816356 7.404405674
ILMN_1224596 7.355131207
ILMN_1233643 7.098400918
ILMN_2808939 9.205580575

Total number of rows: 45281

Table truncated, full table size 1100 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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