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Status |
Public on Jan 08, 2015 |
Title |
Interscapular brown adipose tissue in mice fed High-fat diet 4 weeks rep2 |
Sample type |
RNA |
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Source name |
Interscapular brown adipose_High-fat diet_4 wks
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Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J age: 7 weeks diet: High-fat diet time point: 4 weeks tissue: Interscapular brown adipose tissue
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Treatment protocol |
C57BL/6J mice were fed a high-fat diet (HFD) or normal diet (ND) and sacrificed at 5 time-points (2, 4, 8, 20 and 24 weeks) over 24 weeks.
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Growth protocol |
All the mice were individually housed under a constant temperature (24°C) and 12-hour light/dark cycle, fed the AIN-76 semi-purified diet or AIN-76 semi-purified diet with 39.2 kcal% fat (American Institute of Nutrition, 1977) consisting of 20% fat and 1% cholesterol.
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
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Hybridization protocol |
Standard Illumina hybridization protocol
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Scan protocol |
Standard Illumina scanning protocol
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Description |
replicate 2 B4H2
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Data processing |
Raw data were extracted using the software provided by the manufacturer (Illumina BeadStudio v3.1.3 (Gene Expression Module v3.3.8). The data were normalised using quantile normalisation with beadarray in R
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Submission date |
Jan 07, 2015 |
Last update date |
Jan 08, 2015 |
Contact name |
Sanggyu Lee |
E-mail(s) |
slee@knu.ac.kr
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Organization name |
Kyungpook National University
|
Street address |
1370 Sankyuk-dong, Bukgu
|
City |
Daegu |
ZIP/Postal code |
702-701 |
Country |
South Korea |
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Platform ID |
GPL6887 |
Series (1) |
GSE64718 |
Gene expression profiles reveal the effect of a high-fat diet in brown fat development |
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