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Sample GSM1577838 Query DataSets for GSM1577838
Status Public on Jan 08, 2015
Title Interscapular brown adipose tissue in mice fed High-fat diet 4 weeks rep2
Sample type RNA
 
Source name Interscapular brown adipose_High-fat diet_4 wks
Organism Mus musculus
Characteristics strain: C57BL/6J
age: 7 weeks
diet: High-fat diet
time point: 4 weeks
tissue: Interscapular brown adipose tissue
Treatment protocol C57BL/6J mice were fed a high-fat diet (HFD) or normal diet (ND) and sacrificed at 5 time-points (2, 4, 8, 20 and 24 weeks) over 24 weeks.
Growth protocol All the mice were individually housed under a constant temperature (24°C) and 12-hour light/dark cycle, fed the AIN-76 semi-purified diet or AIN-76 semi-purified diet with 39.2 kcal% fat (American Institute of Nutrition, 1977) consisting of 20% fat and 1% cholesterol.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 2
B4H2
Data processing Raw data were extracted using the software provided by the manufacturer (Illumina BeadStudio v3.1.3 (Gene Expression Module v3.3.8). The data were normalised using quantile normalisation with beadarray in R
 
Submission date Jan 07, 2015
Last update date Jan 08, 2015
Contact name Sanggyu Lee
E-mail(s) slee@knu.ac.kr
Organization name Kyungpook National University
Street address 1370 Sankyuk-dong, Bukgu
City Daegu
ZIP/Postal code 702-701
Country South Korea
 
Platform ID GPL6887
Series (1)
GSE64718 Gene expression profiles reveal the effect of a high-fat diet in brown fat development

Data table header descriptions
ID_REF
VALUE Quantile normalized (log2)

Data table
ID_REF VALUE
ILMN_2417611 6.7890707
ILMN_2762289 7.027309527
ILMN_2896528 13.12743364
ILMN_2721178 11.6573852
ILMN_2458837 6.886165654
ILMN_3033922 11.92418209
ILMN_3092673 13.53097748
ILMN_1230777 12.68657116
ILMN_1246069 14.55087281
ILMN_1232042 6.884758281
ILMN_1243193 10.26157342
ILMN_2524361 6.838884217
ILMN_1242440 6.777234804
ILMN_1233188 7.737588679
ILMN_2543688 10.21665222
ILMN_1259789 7.881446183
ILMN_2816356 7.353567548
ILMN_1224596 7.771832606
ILMN_1233643 7.018492305
ILMN_2808939 8.855092303

Total number of rows: 45281

Table truncated, full table size 1100 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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