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Sample GSM1577839 Query DataSets for GSM1577839
Status Public on Jan 08, 2015
Title Interscapular brown adipose tissue in mice fed High-fat diet 4 weeks rep3
Sample type RNA
 
Source name Interscapular brown adipose_High-fat diet_4 wks
Organism Mus musculus
Characteristics strain: C57BL/6J
age: 7 weeks
diet: High-fat diet
time point: 4 weeks
tissue: Interscapular brown adipose tissue
Treatment protocol C57BL/6J mice were fed a high-fat diet (HFD) or normal diet (ND) and sacrificed at 5 time-points (2, 4, 8, 20 and 24 weeks) over 24 weeks.
Growth protocol All the mice were individually housed under a constant temperature (24°C) and 12-hour light/dark cycle, fed the AIN-76 semi-purified diet or AIN-76 semi-purified diet with 39.2 kcal% fat (American Institute of Nutrition, 1977) consisting of 20% fat and 1% cholesterol.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 3
B4H3
Data processing Raw data were extracted using the software provided by the manufacturer (Illumina BeadStudio v3.1.3 (Gene Expression Module v3.3.8). The data were normalised using quantile normalisation with beadarray in R
 
Submission date Jan 07, 2015
Last update date Jan 08, 2015
Contact name Sanggyu Lee
E-mail(s) slee@knu.ac.kr
Organization name Kyungpook National University
Street address 1370 Sankyuk-dong, Bukgu
City Daegu
ZIP/Postal code 702-701
Country South Korea
 
Platform ID GPL6887
Series (1)
GSE64718 Gene expression profiles reveal the effect of a high-fat diet in brown fat development

Data table header descriptions
ID_REF
VALUE Quantile normalized (log2)

Data table
ID_REF VALUE
ILMN_2417611 6.910451265
ILMN_2762289 7.141362467
ILMN_2896528 13.13192816
ILMN_2721178 11.46156672
ILMN_2458837 6.747719028
ILMN_3033922 11.88753401
ILMN_3092673 13.62571281
ILMN_1230777 12.52575795
ILMN_1246069 14.44527011
ILMN_1232042 6.715603326
ILMN_1243193 10.34975555
ILMN_2524361 6.910248854
ILMN_1242440 6.798776551
ILMN_1233188 7.484366619
ILMN_2543688 10.46780795
ILMN_1259789 7.778913305
ILMN_2816356 7.523716606
ILMN_1224596 7.589990974
ILMN_1233643 7.015645771
ILMN_2808939 8.861250543

Total number of rows: 45281

Table truncated, full table size 1100 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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