|
| Status |
Public on Jan 08, 2015 |
| Title |
Interscapular brown adipose tissue in mice fed High-fat diet 20 weeks rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Interscapular brown adipose_High-fat diet_20 wks
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
age: 7 weeks
diet: High-fat diet
time point: 20 weeks
tissue: Interscapular brown adipose tissue
|
| Treatment protocol |
C57BL/6J mice were fed a high-fat diet (HFD) or normal diet (ND) and sacrificed at 5 time-points (2, 4, 8, 20 and 24 weeks) over 24 weeks.
|
| Growth protocol |
All the mice were individually housed under a constant temperature (24°C) and 12-hour light/dark cycle, fed the AIN-76 semi-purified diet or AIN-76 semi-purified diet with 39.2 kcal% fat (American Institute of Nutrition, 1977) consisting of 20% fat and 1% cholesterol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
B20H3
|
| Data processing |
Raw data were extracted using the software provided by the manufacturer (Illumina BeadStudio v3.1.3 (Gene Expression Module v3.3.8). The data were normalised using quantile normalisation with beadarray in R
|
| |
|
| Submission date |
Jan 07, 2015 |
| Last update date |
Jan 08, 2015 |
| Contact name |
Sanggyu Lee |
| E-mail(s) |
slee@knu.ac.kr
|
| Organization name |
Kyungpook National University
|
| Street address |
1370 Sankyuk-dong, Bukgu
|
| City |
Daegu |
| ZIP/Postal code |
702-701 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE64718 |
Gene expression profiles reveal the effect of a high-fat diet in brown fat development |
|
