|
| Status |
Public on Jan 26, 2015 |
| Title |
Zebrafish cardiac tissue 72 hpf ccm2 mutant sample 1 |
| Sample type |
RNA |
| |
|
| Source name |
cardiac tissue, pooled from embryo extractions 72 hpf, ccm2 m201 mutant
|
| Organism |
Danio rerio |
| Characteristics |
tissue: cardiac tissue
genotype: ccm2 m201 mutant
|
| Treatment protocol |
ccm2 m201 mutant and WT embryos were raised to 72 hpf.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole hearts were extracted manually from 72 hpf embryos as described in Burns and MacRae, 2006. Total RNA was extracted with RNeasy Micro Kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
For each sample, 200ng of intact (RIN > 8) total RNA was subjected to a single amplification step and Cy3 labeling reaction.
|
| |
|
| Hybridization protocol |
1.5 µg of labeled cRNA were hybridized to Zebrafish (V2) Gene Expression Microarrays (G2519F-019161, 4x44K)
|
| Scan protocol |
Spot intensities were recorded on a G2565BA scanner (Agilent Technologies)
|
| Description |
cardiac gene expression after Bmp2b overexpression
251916110291 subarray 1_3
|
| Data processing |
Scanned images were analyzed after all the Feature Extraction processing steps were completed. In the case of one color, Processsed Signal contains the Multiplicatively Detrended Background Subtracted Signal if the detrending is selected and helps. If the detrending does not help, this column will contain the Background Subtracted Signal.
|
| |
|
| Submission date |
Jan 07, 2015 |
| Last update date |
Jan 26, 2015 |
| Contact name |
Salim Abdelilah-Seyfried |
| E-mail(s) |
salim.seyfried@uni-potsdam.de
|
| Phone |
+49 (331) 9775540
|
| Organization name |
University of Potsdam
|
| Department |
Animal Physiology
|
| Street address |
Karl-Liebknecht Strasse 24-25, Bldg. 26
|
| City |
Potsdam |
| ZIP/Postal code |
14476 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6457 |
| Series (1) |
| GSE64753 |
CCm2-regulated cardiac genes in zebrafish |
|
