|
| Status |
Public on Jan 26, 2016 |
| Title |
sh Chd9 [5506502022_C] |
| Sample type |
RNA |
| |
|
| Source name |
Mouse ES cells
|
| Organism |
Mus musculus |
| Characteristics |
transfection: shRNA Chd9
sample id: LT-82
|
| Treatment protocol |
The pHYPER shRNA vectors were transfected in embryonic stem cells cell by electroporation. 24 h after transfection, puromycin selection was applied for an additional 48 h period, before cell collection and RNA preparation
|
| Growth protocol |
Embryonic stem cells were cultured at 37°C, 5% CO2, on mitomycin C-inactivated mouse embryonic fibroblasts, in DMEM (Sigma) with 15% foetal bovine serum and a saturating amount of leukemia inhibitory factor (LIF)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using an RNeasy Kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 4
|
| Data processing |
The data were normalised using quantile normalisation with GenomeStudio
|
| |
|
| Submission date |
Jan 08, 2015 |
| Last update date |
Jan 27, 2016 |
| Contact name |
Robert Olaso |
| E-mail(s) |
olaso@cnrgh.fr
|
| Phone |
33(1)601632112
|
| Organization name |
CEA-DRF
|
| Department |
CNRGH
|
| Lab |
L2PGH
|
| Street address |
2 rue Gaston Cremieux
|
| City |
Evry Cedex |
| ZIP/Postal code |
91057 |
| Country |
France |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE64785 |
Genome wide nucleosome specifity and function of chromatin remodellers in embryonic stem cells [Chd9] |
| GSE64819 |
Genome wide nucleosome specifity and function of chromatin remodellers in embryonic stem cells |
|
