|
Status |
Public on Jan 27, 2007 |
Title |
iDC hypoxia 8 |
Sample type |
RNA |
|
|
Source name |
Monocyte derived dendritic cells under hypoxia conditions
|
Organism |
Homo sapiens |
Characteristics |
Monocyte derived from PBMC of healthy donor
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was independently isolated from DCs derived from the Mn of three different donors using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.
|
Label |
biotin
|
Label protocol |
The RNA was reverse-transcribed into cDNA and biotin labeled according to the Affymetrix’s instructions.
|
|
|
Hybridization protocol |
Fragmented cRNA was used for hybridization to Affymetrix HG-U133 plus 2.0 arrays according to the Affymetrix’s instructions.
|
Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner 3000.
|
Description |
Human peripheral blood mononuclear cells (PBMC) were isolated from venous blood of voluntary healthy donors. All cultures were maintained in RPMI 1640 medium supplemented with 10% FBS heat inactivated, 50μg/ml gentamycin. Monocytes were purified by negative selection. To generate IDC the monocytes were incubated in six-well culture plates for 4 days under hypoxic conditions. The monocytes were incubated in six-well culture plates for 4 days under hypoxic conditions.
|
Data processing |
Expression values were quantified and array quality control was performed with the statistical algorithms implemented in Affymetrix Microarray Suite 5.0.
|
|
|
Submission date |
Jan 25, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Paolo Fardin |
E-mail(s) |
paolofardin@ospedale-gaslini.ge.it
|
Organization name |
Istiuto Giannina Gaslini
|
Department |
Molecular Biology
|
Street address |
Largo G. Gaslini 5
|
City |
Genoa |
ZIP/Postal code |
16147 |
Country |
Italy |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE6863 |
Immature dendritic cells under hypoxic condition |
|
Relations |
Reanalyzed by |
GSE64985 |
Reanalyzed by |
GSE86362 |
Reanalyzed by |
GSE119087 |