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Sample GSM158183 Query DataSets for GSM158183
Status Public on Jan 27, 2007
Title iDC hypoxia 8
Sample type RNA
 
Source name Monocyte derived dendritic cells under hypoxia conditions
Organism Homo sapiens
Characteristics Monocyte derived from PBMC of healthy donor
Extracted molecule total RNA
Extraction protocol Total RNA was independently isolated from DCs derived from the Mn of three different donors using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.
Label biotin
Label protocol The RNA was reverse-transcribed into cDNA and biotin labeled according to the Affymetrix’s instructions.
 
Hybridization protocol Fragmented cRNA was used for hybridization to Affymetrix HG-U133 plus 2.0 arrays according to the Affymetrix’s instructions.
Scan protocol GeneChips were scanned using an Affymetrix GeneChip Scanner 3000.
Description Human peripheral blood mononuclear cells (PBMC) were isolated from venous blood of voluntary healthy donors. All cultures were maintained in RPMI 1640 medium supplemented with 10% FBS heat inactivated, 50μg/ml gentamycin. Monocytes were purified by negative selection. To generate IDC the monocytes were incubated in six-well culture plates for 4 days under hypoxic conditions.
The monocytes were incubated in six-well culture plates for 4 days under hypoxic conditions.
Data processing Expression values were quantified and array quality control was performed with the statistical algorithms implemented in Affymetrix Microarray Suite 5.0.
 
Submission date Jan 25, 2007
Last update date Aug 28, 2018
Contact name Paolo Fardin
E-mail(s) paolofardin@ospedale-gaslini.ge.it
Organization name Istiuto Giannina Gaslini
Department Molecular Biology
Street address Largo G. Gaslini 5
City Genoa
ZIP/Postal code 16147
Country Italy
 
Platform ID GPL570
Series (1)
GSE6863 Immature dendritic cells under hypoxic condition
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE86362
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE signal intensity
ABS_CALL flag
DETECTION P-VALUE statistical significance

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 641.3208 P 7.54E-04
AFFX-BioB-M_at 971.4576 P 8.14E-05
AFFX-BioB-3_at 467.43643 P 5.09E-04
AFFX-BioC-5_at 1690.3867 P 1.10E-04
AFFX-BioC-3_at 1984.9346 P 4.43E-05
AFFX-BioDn-5_at 3108.3706 P 5.17E-05
AFFX-BioDn-3_at 8583.777 P 3.40E-04
AFFX-CreX-5_at 21277.41 P 5.17E-05
AFFX-CreX-3_at 24844.88 P 4.43E-05
AFFX-DapX-5_at 66.00088 A 0.13136113
AFFX-DapX-M_at 5.092077 A 0.6994095
AFFX-DapX-3_at 4.1512723 A 0.8764277
AFFX-LysX-5_at 21.497755 A 0.48510987
AFFX-LysX-M_at 43.715317 A 0.51489013
AFFX-LysX-3_at 24.732193 A 0.14793876
AFFX-PheX-5_at 6.048988 A 0.7834759
AFFX-PheX-M_at 4.1919622 A 0.9370707
AFFX-PheX-3_at 25.01053 A 0.5886198
AFFX-ThrX-5_at 25.532038 A 0.54459137
AFFX-ThrX-M_at 16.752153 A 0.6315623

Total number of rows: 54675

Table truncated, full table size 1693 Kbytes.




Supplementary file Size Download File type/resource
GSM158183.CEL.gz 5.4 Mb (ftp)(http) CEL

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