|
| Status |
Public on Oct 01, 2015 |
| Title |
BE tumor from patient 4 BE tumor Pt4-3 |
| Sample type |
RNA |
| |
|
| Source name |
Transformed Barett's esophagus stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Cultured stem cells transformed with hTERT, SV40 and c-myc
cell type: Barett's esophagus stem cells
|
| Treatment protocol |
Stem cells were transformed with retroviral infection of hTERT, SV40 and c-myc and then subcutaneously transplanted into highly immunocompromised NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
3' IVT PLUS Kit (affymetrix)
|
| |
|
| Hybridization protocol |
GeneChip® Hybridization, Wash, and Stain Kit (affymetrix)
|
| Scan protocol |
GeneChip® Hybridization, Wash, and Stain Kit (affymetrix)
|
| Description |
Gene expression data from xenografted tumor
Barett's esophagus stem cells
|
| Data processing |
Expression console, Partek Genome Suite 6.6
|
| |
|
| Submission date |
Jan 12, 2015 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Frank McKeon |
| E-mail(s) |
mckeon.xian@gmail.com
|
| Phone |
8608372486
|
| Organization name |
University of Houston
|
| Department |
Biology and Biochemistry
|
| Street address |
3455 Cullen Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77004 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE64894 |
Transformation of Barrett's esophagus stem cell, gastric cardia stem cells and normal esophagus stem cells |
|
