|
| Status |
Public on Apr 26, 2007 |
| Title |
SREBP1c vs mock infected, rep3 (dye swap) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Human Fibroblasts cells AG01518
|
| Organism |
Homo sapiens |
| Characteristics |
Human Fibroblasts cells AG01518 infected with mock viruses
|
| Treatment protocol |
AG01518 fibroblasts were infected with adenoviruses encoding mock virus at 60 virus per cell for 24 h
|
| Growth protocol |
AG01518 foreskin fibroblasts from passages 11 to 19 were cultured in Modified Eagle Medium supplemented with 10% foetal calf serum and glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA (40 ug) was isolated from cells infected with mock viruses using the RNeasy kit (Qiagen) and used for microarray hybridizations
|
| Label |
Cy5
|
| Label protocol |
total RNA was labeled with Cy5
|
| |
|
| Channel 2 |
| Source name |
Human Fibroblasts cells AG01518
|
| Organism |
Homo sapiens |
| Characteristics |
Human Fibroblasts cells AG01518 infected with SREBP1c encoding viruses
|
| Treatment protocol |
AG01518 fibroblasts were infected with adenoviruses encoding SREBP1c at 60 virus per cell for 24 h
|
| Growth protocol |
AG01518 foreskin fibroblasts from passages 11 to 19 were cultured in Modified Eagle Medium supplemented with 10% foetal calf serum and glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA (40 ug) was isolated from cells infected with SREBP1c encoding viruses using the RNeasy kit (Qiagen) and used for microarray hybridizations
|
| Label |
Cy3
|
| Label protocol |
total RNA was labeled with Cy3
|
| |
|
| |
| Hybridization protocol |
Purified cDNA probes labelled with Cy3 and Cy5 were mixed per pair, and hybridized to cDNA microarray chips (hver1.2.1) from the Sanger Institute/LICR/CRUK Consortium
|
| Scan protocol |
Chips were scanned in a Perkin Elmer/GSI Lumonics ScanArray 4000 scanner and spot intensities were measured using the QuantArray software (histogram method with background subtraction)
|
| Description |
Human Fibroblast cells(AG01518) infected with adenovirus coding for SREBP1c and mock virus as control
|
| Data processing |
Normalization and statistical analysis of the data sets were performed using GeneSpring 5.0. A Lowess non-linear normalization was applied, and a statistical analysis of the data based on the global error model of GeneSpring was used
|
| |
|
| Submission date |
Jan 26, 2007 |
| Last update date |
Apr 26, 2007 |
| Contact name |
Jean-Baptiste Demoulin |
| Organization name |
Université catholique de Louvain
|
| Department |
de Duve Institute
|
| Lab |
UCL - MEXP
|
| Street address |
av Hippocrate 75, B1.74.05
|
| City |
Brussels |
| ZIP/Postal code |
B-1200 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL506 |
| Series (1) |
| GSE6877 |
SREBP1c and mock virus expression profiles in AG01518 human fibroblasts |
|
