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Sample GSM1586723 Query DataSets for GSM1586723
Status Public on Feb 26, 2015
Title TGFB1+TAK1,biological rep1
Sample type RNA
 
Source name TGFB1+TAK1,biological
Organism Homo sapiens
Characteristics cell type: gingival fibroblast
treatment: TBFB1 + 5Z-7-Oxozeanol
Treatment protocol Cells were pre-treated with DMSO or 400 nM (5Z)-7-Oxozeanol for 45 minutes prior to treatment with or without TGFβ1 (4 ng/ml, R&D systems) for 6 hours
Growth protocol Gingival fibroblasts were grown in DMEM 10% FBS until 40-60% confluence and then in DMEM 0.5% FBS overnight prior to treatment
Extracted molecule total RNA
Extraction protocol TRIZOL was used to extract RNA
Label biotin
Label protocol Biotin end labeled single strand cDNA was prepared according to the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay Manual
 
Hybridization protocol 5.5 ug of single stranded cDNA was hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Data processing The data were analyzed with Partek Genomcis Suite using the RMA normalization method
 
Submission date Jan 16, 2015
Last update date Sep 01, 2016
Contact name David E Carter
E-mail(s) dcarter@robarts.ca
Organization name Robarts Research Institute
Department London Regional Genomics Centre
Street address 1151 Richmond Street North
City London
State/province ON
ZIP/Postal code N6A5B7
Country Canada
 
Platform ID GPL6244
Series (1)
GSE65069 5Z-7-Oxozeanol inhibits the effect of TGFB1 on human gingival fibroblasts
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
7892501 5.10971
7892502 5.49414
7892503 3.91428
7892504 10.4346
7892505 4.86118
7892506 5.10709
7892507 6.52416
7892508 6.22819
7892509 11.7606
7892510 5.97587
7892511 5.45093
7892512 8.15313
7892513 4.43758
7892514 11.7085
7892515 10.7275
7892516 4.85362
7892517 7.04707
7892518 4.39285
7892519 5.46793
7892520 10.1878

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM1586723_E1348.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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