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Sample GSM1586724 Query DataSets for GSM1586724
Status Public on Feb 26, 2015
Title DMSO,biological rep2
Sample type RNA
 
Source name DMSO,biological
Organism Homo sapiens
Characteristics cell type: gingival fibroblast
treatment: DMSO
Treatment protocol Cells were pre-treated with DMSO or 400 nM (5Z)-7-Oxozeanol for 45 minutes prior to treatment with or without TGFβ1 (4 ng/ml, R&D systems) for 6 hours
Growth protocol Gingival fibroblasts were grown in DMEM 10% FBS until 40-60% confluence and then in DMEM 0.5% FBS overnight prior to treatment
Extracted molecule total RNA
Extraction protocol TRIZOL was used to extract RNA
Label biotin
Label protocol Biotin end labeled single strand cDNA was prepared according to the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay Manual
 
Hybridization protocol 5.5 ug of single stranded cDNA was hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Data processing The data were analyzed with Partek Genomcis Suite using the RMA normalization method
 
Submission date Jan 16, 2015
Last update date Sep 01, 2016
Contact name David E Carter
E-mail(s) dcarter@robarts.ca
Organization name Robarts Research Institute
Department London Regional Genomics Centre
Street address 1151 Richmond Street North
City London
State/province ON
ZIP/Postal code N6A5B7
Country Canada
 
Platform ID GPL6244
Series (1)
GSE65069 5Z-7-Oxozeanol inhibits the effect of TGFB1 on human gingival fibroblasts
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
7892501 4.56604
7892502 5.41509
7892503 3.82724
7892504 10.1082
7892505 4.96615
7892506 5.41623
7892507 6.67788
7892508 6.90804
7892509 11.7196
7892510 5.37338
7892511 4.94639
7892512 8.34472
7892513 4.78898
7892514 11.1533
7892515 10.4681
7892516 4.79778
7892517 7.05265
7892518 4.18414
7892519 5.40183
7892520 10.0512

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM1586724_E1355.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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