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Sample GSM1586726 Query DataSets for GSM1586726
Status Public on Feb 26, 2015
Title TGFB1,biological rep2
Sample type RNA
 
Source name TGFB1,biological
Organism Homo sapiens
Characteristics cell type: gingival fibroblast
treatment: TBFB2
Treatment protocol Cells were pre-treated with DMSO or 400 nM (5Z)-7-Oxozeanol for 45 minutes prior to treatment with or without TGFβ1 (4 ng/ml, R&D systems) for 6 hours
Growth protocol Gingival fibroblasts were grown in DMEM 10% FBS until 40-60% confluence and then in DMEM 0.5% FBS overnight prior to treatment
Extracted molecule total RNA
Extraction protocol TRIZOL was used to extract RNA
Label biotin
Label protocol Biotin end labeled single strand cDNA was prepared according to the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay Manual
 
Hybridization protocol 5.5 ug of single stranded cDNA was hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Data processing The data were analyzed with Partek Genomcis Suite using the RMA normalization method
 
Submission date Jan 16, 2015
Last update date Sep 01, 2016
Contact name David E Carter
E-mail(s) dcarter@robarts.ca
Organization name Robarts Research Institute
Department London Regional Genomics Centre
Street address 1151 Richmond Street North
City London
State/province ON
ZIP/Postal code N6A5B7
Country Canada
 
Platform ID GPL6244
Series (1)
GSE65069 5Z-7-Oxozeanol inhibits the effect of TGFB1 on human gingival fibroblasts
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
7892501 4.28108
7892502 5.27221
7892503 4.07285
7892504 10.1233
7892505 4.6113
7892506 5.05997
7892507 7.06297
7892508 5.98917
7892509 11.8456
7892510 4.52107
7892511 5.3102
7892512 8.21098
7892513 4.65711
7892514 11.4214
7892515 10.8393
7892516 5.1942
7892517 6.89693
7892518 3.62337
7892519 5.43759
7892520 10.1465

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM1586726_E1357.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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