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Sample GSM1586727 Query DataSets for GSM1586727
Status Public on Feb 26, 2015
Title TGFB1+TAK1,biological rep2
Sample type RNA
 
Source name TGFB1+TAK1,biological
Organism Homo sapiens
Characteristics cell type: gingival fibroblast
treatment: TBFB1 + 5Z-7-Oxozeanol
Treatment protocol Cells were pre-treated with DMSO or 400 nM (5Z)-7-Oxozeanol for 45 minutes prior to treatment with or without TGFβ1 (4 ng/ml, R&D systems) for 6 hours
Growth protocol Gingival fibroblasts were grown in DMEM 10% FBS until 40-60% confluence and then in DMEM 0.5% FBS overnight prior to treatment
Extracted molecule total RNA
Extraction protocol TRIZOL was used to extract RNA
Label biotin
Label protocol Biotin end labeled single strand cDNA was prepared according to the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay Manual
 
Hybridization protocol 5.5 ug of single stranded cDNA was hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Data processing The data were analyzed with Partek Genomcis Suite using the RMA normalization method
 
Submission date Jan 16, 2015
Last update date Sep 01, 2016
Contact name David E Carter
E-mail(s) dcarter@robarts.ca
Organization name Robarts Research Institute
Department London Regional Genomics Centre
Street address 1151 Richmond Street North
City London
State/province ON
ZIP/Postal code N6A5B7
Country Canada
 
Platform ID GPL6244
Series (1)
GSE65069 5Z-7-Oxozeanol inhibits the effect of TGFB1 on human gingival fibroblasts
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
7892501 5.26426
7892502 5.32727
7892503 4.06517
7892504 9.94171
7892505 3.55056
7892506 4.63272
7892507 6.45119
7892508 4.87297
7892509 11.7518
7892510 5.19477
7892511 5.2083
7892512 7.87548
7892513 4.02532
7892514 11.5522
7892515 10.728
7892516 4.97011
7892517 7.10468
7892518 4.11878
7892519 5.31112
7892520 10.0103

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM1586727_E1358.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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