|
| Status |
Public on Jan 22, 2015 |
| Title |
786-O VHL biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
renal cell carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 786-O
transfection: VHL
condition: normoxia
|
| Treatment protocol |
No treatment
|
| Growth protocol |
3 x 10^6 786-O VHL-negative and VHL-transfected cells were incubated for 24 h during normoxia or hypoxia conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent (Invitrogen), reverse transcribed and amplified with the WT expression kit (Invitrogen) according to the manufacturer’s manual.
|
| Label |
biotin
|
| Label protocol |
Fragmented cDNA was end-labeled using Affymetrix terminal labeling kit .
|
| |
|
| Hybridization protocol |
Labeled cDNA was hybridized onto Human GeneChip 1.0 ST arrays (Affymetrix). The chips were stained and washed following standard procedure.
|
| Scan protocol |
The chips were scanned on a GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
gene expression data of 786-O cells transfected with a VHL-overexpression vector incubated during normoxia
|
| Data processing |
Data analyses were performed using Partek Genomic Suite (Partek) software after Robust Multichip Average (RMA) normalization.
|
| |
|
| Submission date |
Jan 21, 2015 |
| Last update date |
Jan 22, 2015 |
| Contact name |
Anja Harder |
| E-mail(s) |
anja.harder@uk-halle.de
|
| Organization name |
Martin Luther University Halle-Wittenberg
|
| Department |
Cure NF Research Group
|
| Street address |
Große Steinstr. 52
|
| City |
Halle (Saale) |
| ZIP/Postal code |
06108 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE65168 |
Cellular and molecular characterization of the altered metabolism in RCC |
|
