|
| Status |
Public on Jan 24, 2015 |
| Title |
HCC38_6h-Wnt3a_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HCC38
|
| Organism |
Homo sapiens |
| Characteristics |
wnt3a treatment: no
time after treatment (hours): 6
cell line: HCC38
|
| Treatment protocol |
Cells were seeded in six-well plates, serum starved overnight, and then treated with Wnt3a for the indicated times (6, 12 or 24 hours). Triplicates for each condition were included in the experiment.
|
| Growth protocol |
HCC38 and MDA-MB-468 cells were obtained from the American Type Culture Collection and authenticated in 2013 by short tandem repeat profiling. MDA-MB-468 cells were maintained in RPMI-1640 containing Glutamax and supplemented with 10% FBS. HCC38 cells were cultured in RPMI-1640 with Glutamax containing 10% FBS, 1.5g/L sodium bicarbonate, 10mM Hepes and 1mM sodium pyruvate. Cells were cultured at 37°C in a 5% CO2 humidified incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the RNeasy Mini Kit from Qiagen (Courtaboeuf, France) following the manufacturer’s recommendations.
|
| Label |
biotin
|
| Label protocol |
After RNA quality and quantity controls, samples were hybridized onto Gene st 1.1 Affymetrix chips. Samples were processed as described on the website of the company.
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
The data were analyzed with the brainarray HuGene11stv1_Hs_ENTREZG version 14 custom chipset definition file for the HuGene11stv1 affymetrix array. The data were first log2 transformed and normalized with RMA.
|
| |
|
| Submission date |
Jan 23, 2015 |
| Last update date |
Jan 24, 2015 |
| Contact name |
Thierry Dubois |
| E-mail(s) |
thierry.dubois@curie.fr
|
| Organization name |
Institut Curie
|
| Department |
Recherche Center
|
| Lab |
Breast Cancer Biology Group
|
| Street address |
26 rue d’Ulm
|
| City |
PARIS |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL15640 |
| Series (1) |
| GSE65238 |
Transcriptome analysis of Wnt3a-treated triple-negative breast cancer cell lines. |
|
