|
| Status |
Public on Nov 04, 2015 |
| Title |
rasV12FRT82Bscrib1_2 |
| Sample type |
SRA |
| |
|
| Source name |
Mosaic eye/antennal imaginal discs
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: epithelial cells
tissue: eye/antennal imaginal discs
age: 3d instar larval stage
genotype/variation: rasV12FRT82Bscrib1
|
| Growth protocol |
MARCM fly crosses were carried out at 25°C on standard media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from eye/antennal imaginal discs dissected from third-instar larvae with Isol-RNA Lysis Reagent (5 Prime).
Sequencing libraries were generated according to the Illumina protocol for total RNA library preparation and loaded onto Illumina flow cells (Illumina, San Diego, CA)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Illumina Casava 1.8.2 software used for basecalling
RNAseq reads were aligned drosophila melanogaster
Fragments Per Kilobase of exon per Million mapped fragments (FPKM) were calculated using a Cufflinks
Genome_build: drosophila melanogaster 75
Supplementary_files_format_and_content: Excel file reports FPKMs for a given sample.
Supplementary_files_format_and_content: Table_S1_transcriptome_analysis.xlsx (linked as supplementary file on Series record) reports fold-change between samples.
|
| |
|
| Submission date |
Jan 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Mirka Uhlirova |
| E-mail(s) |
mirka.uhlirova@uni-koeln.de
|
| Organization name |
CECAD Research Center Cologne
|
| Department |
Institute for Genetics
|
| Lab |
Uhlirova Lab
|
| Street address |
Joseph-Stelzmann Str. 26
|
| City |
Cologne |
| State/province |
NRW |
| ZIP/Postal code |
50931 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13304 |
| Series (1) |
| GSE65261 |
Ets-domain transcription factor Ets21c and nuclear receptor Ftz-F1 are novel effectors of JNK promoting tumor malignancy |
|
| Relations |
| Reanalyzed by |
GSM3287743 |
| BioSample |
SAMN03294329 |
| SRA |
SRX852473 |
