|
| Status |
Public on Mar 19, 2007 |
| Title |
Rice coleoptiles, 4 days old, aerobic, replicate N.1 (sample#9) |
| Sample type |
RNA |
| |
|
| Source name |
Rice coleoptiles, 4 days old, aerobic
|
| Organism |
Oryza sativa |
| Characteristics |
Rice coleoptiles, free of leaf tissue. Rice cultivar: Nipponbare Age: 4 days Germination: aerobic, 28°C, darkness
|
| Treatment protocol |
This is the control: aerobic samples to be compared to anoxic samples
|
| Growth protocol |
Rice (Oryza sativa L. cv Nipponbare) seeds were sterilized with diluted bleach (15-min incubation in 1.7% sodium hypochlorite, rinsing and washing in sterile water 10 times) and germinated for four days in air on sterile filter paper at 28°C in the dark. The primary leaf was removed from the coleoptiles of air-germinated seedlings (the leaf is absent in anoxia-germinated seedlings). All the air/anoxia treatments were performed in the dark at 28°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the seedling samples using the Ambion RNAqueous kit (Ambion). RNA quality was assessed by agarose gel electrophoresis and spectrophotometry. RNA was processed for use on Affymetrix Rice Genome GeneChip arrays.
|
| Label |
Biotin
|
| Label protocol |
Affymetrix standard labeling protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard hybridization protocol
|
| Scan protocol |
Affymetrix standard scan protocol
|
| Description |
no additional description required
|
| Data processing |
GC-RMA algorithm
|
| |
|
| Submission date |
Jan 29, 2007 |
| Last update date |
Mar 19, 2007 |
| Contact name |
Pierdomenico Perata |
| E-mail(s) |
p.perata@sssup.it
|
| Phone |
+39050881901
|
| Organization name |
Sant'Anna School of Advanced Studies
|
| Department |
Institute of Life Sciences
|
| Lab |
PlantLab
|
| Street address |
Via Guidiccioni 8-10
|
| City |
San Giuliano Terme |
| State/province |
Pisa |
| ZIP/Postal code |
56010 |
| Country |
Italy |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE6908 |
Transcript Profiling of the Aerobic and Anoxic Rice Coleoptile |
|
