See reference:Neoh HM, et al.: Mutated response regulator graR is responsible for phenotypic conversion of Staphylococcus aureus from heterogeneous vancomycin-intermediate resistance to vancomycin-intermediate resistance. Antimicrob Agents Chemother. 2008 Jan;52(1):45-53.
Label
Cy3
Label protocol
SuperScript Indirect cDNA Labeling System (Invitrogen Life Technologies, Carlsbad, California).
Hybridization protocol
Labeled samples were hybridized onto the microarray at 50°C for 16-20 hr. After hybridization, the arrays were washed in the following buffers, and dry by spin slide. 2X SSC/0.05% SDS, for 15 min 0.2X SSC/0.05% SDS, for 15 min 0.5X SSC, for 3 min 0.2X SSC, for 3 min
