|
| Status |
Public on Jun 29, 2007 |
| Title |
R. palustris hydrogen-producing mutant (CGA584) Replicate #1 |
| Sample type |
RNA |
| |
|
| Source name |
Photosynthetic bacterium Rhodopseudomonas palustris
|
| Organism |
Rhodopseudomonas palustris |
| Characteristics |
R. palustris hydrogen-producing mutant (CGA584) Replicate #1
|
| Growth protocol |
Photosynthetically grown with acetate and with ammonium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rhodopseudomonas palustris cells were disrupted by treatment with by bead beating and RNA was purified with the RNeasy Mini kit (Qiagen) including DNase treatment on the column.
|
| Label |
Biotinylated GeneChip DNA labeling reagent
|
| Label protocol |
As recommended by the manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
As recommended by the manufacturer (Affymetrix)
|
| Scan protocol |
As recommended by the manufacturer (Affymetrix)
|
| Description |
A high-density oligonucleotide microarray (Affymetrix GeneChip) was custom designed and manufactured by Affymetrix based on the sequences of ORFs and intergenic regions. cDNA synthesis, fragmentation, labeling, hybridization, and processing of custom designed Rhodopseudomonas palustris GeneChips were carried out as recommended by the manufacturer. Experiments were performed in duplicate with independently prepared samples.
|
| Data processing |
The Affymetrix Microarray Suite was used for initial data acquisition and processing.
|
| |
|
| Submission date |
Jan 29, 2007 |
| Last update date |
Feb 05, 2007 |
| Contact name |
Yasuhiro Oda |
| E-mail(s) |
yasuhiro@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Department of Microbiology
|
| Lab |
Harwood
|
| Street address |
Box 357242, 1705 N.E. Pacific Street, HSB K-354
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195-7242 |
| Country |
USA |
| |
|
| Platform ID |
GPL3954 |
| Series (1) |
| GSE5194 |
Redirection of metabolism for biological hydrogen production |
|
