|
| Status |
Public on Oct 01, 2015 |
| Title |
M3O-lenti-iPSCs-LIF(-) A |
| Sample type |
RNA |
| |
|
| Source name |
Cell Line
|
| Organism |
Mus musculus |
| Characteristics |
cell type: iPSCs
leukemia inhibitory factor (lif) treatment: -
|
| Treatment protocol |
LIF was omitted during establishment and maintenance of M3O-lenti-iPSCs-LIF(–).
|
| Growth protocol |
ESCs and iPSCs-LIF(+) were cultured on irradiated MEF feeder cells in iPSC medium (DMEM, 20% FBS or KnockOut Serum Replacement [Life Technologies], 100 µM MEM non-essential amino acids, 100 µM 2-mercaptoethanol, and 2 mM L-glutamine with or without 1,000 U/ml LIF)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from each cell type with a PureLink RNA Mini Kit (Life Technologies).
|
| Label |
biotin
|
| Label protocol |
Samples with an RNA Integrity Number of 7.5 or greater measured with a LabChip Caliper GX (Perkin Elmer) were applied to an Illumina TotalPrep-96 RNA Amplification Kit (Life Technologies) to generate biotinylated and amplified RNA.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Sample name: M6
|
| Data processing |
The obtained data were analyzed with an Illumina Genome Studio.
The data were normalized using rank invariant method.
|
| |
|
| Submission date |
Feb 03, 2015 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Nobuaki Kikyo, Hiroyuki Hirai |
| E-mail(s) |
kikyo001@umn.edu, hiraih@umich.edu
|
| Organization name |
University of Minnesota
|
| Department |
Genetics, Cell Biology and Development
|
| Street address |
2001 6th St SE
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE65563 |
Genome-wide identification and analysis of mRNA expression in MEFs, ESCs, and iPSCs |
| GSE65597 |
Genome-wide identification and analysis of mRNA and miRNA expression in MEFs, ESCs, and iPSCs |
|
