|
| Status |
Public on Oct 01, 2015 |
| Title |
MEFs-2 |
| Sample type |
RNA |
| |
|
| Source name |
Cell Line
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic fibroblasts
|
| Treatment protocol |
LIF was omitted during establishment and maintenance of M3O-lenti-iPSCs-LIF(–).
|
| Growth protocol |
ESCs and iPSCs-LIF(+) were cultured on irradiated MEF feeder cells in iPSC medium (DMEM, 20% FBS or KnockOut Serum Replacement [Life Technologies], 100 µM MEM non-essential amino acids, 100 µM 2-mercaptoethanol, and 2 mM L-glutamine with or without 1,000 U/ml LIF)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from each cell type with a mirVana miRNA Isolation Kit (Life Technologies).
|
| Label |
Cy5
|
| Label protocol |
Two micrograms of total RNA was extended at the 3′ terminus with a poly(A) tail using poly(A) polymerase. An oligonucleotide tag was then ligated to the poly(A) tail for later fluorescent dye staining.
|
| |
|
| Hybridization protocol |
Hybridization was performed overnight on a µParaflo microfluidic chip using a microcirculation pump (Atactic Technologies).
|
| Scan protocol |
Fluorescence images were collected by using a laser scanner (GenePix 4000B, Molecular Device) and digitized using Array-Pro image analysis software (Media Cybernetics).
|
| Description |
Sample name: M7
|
| Data processing |
Data were analyzed by first subtracting the background and then normalizing the signals using a LOWESS filter (Locally-weighted Regression).
|
| |
|
| Submission date |
Feb 04, 2015 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Nobuaki Kikyo, Hiroyuki Hirai |
| E-mail(s) |
kikyo001@umn.edu, hiraih@umich.edu
|
| Organization name |
University of Minnesota
|
| Department |
Genetics, Cell Biology and Development
|
| Street address |
2001 6th St SE
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL15216 |
| Series (2) |
| GSE65596 |
Genome-wide identification and analysis of microRNA expression in MEFs, ESCs, and iPSCs |
| GSE65597 |
Genome-wide identification and analysis of mRNA and miRNA expression in MEFs, ESCs, and iPSCs |
|
