|
| Status |
Public on Jan 01, 2016 |
| Title |
28DPA grain |
| Sample type |
SRA |
| |
|
| Source name |
Grain
|
| Organism |
Triticum aestivum |
| Characteristics |
variety: Chinese spring
tissue: grain
developmental stage: 28 days post anthesis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the frozen 7, 14, 21 and 28 DPA grains using the ConcertTM Plant RNA Reagent (Invitrogen) according to the manufacturer’s instructions.
Small RNA libraries were prepared for sequencing according to illumina TruSeq Small RNA Sample Preparation Guide.
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Illumina Casava1.5 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and low-quality reads and other contaminants were removed.
Supplementary_files_format_and_content: tab-delimited text files include read counts and length for each sequence
|
| |
|
| Submission date |
Feb 10, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Tian Li |
| E-mail(s) |
litian@caas.cn
|
| Phone |
+86-010-82105826
|
| Organization name |
Institute of Crop Science
|
| Street address |
Chinese Academy of Agricultural Sciences
|
| City |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
| |
|
| Platform ID |
GPL17701 |
| Series (2) |
| GSE65797 |
Expression profiling of small RNAs in developing wheat grains |
| GSE65799 |
Small RNA and degradome sequencing reveal complex roles of miRNAs and their targets in the developing grains of wheat (Triticum aestivum L.) |
|
| Relations |
| BioSample |
SAMN03339257 |
| SRA |
SRX871399 |
