|
| Status |
Public on Dec 31, 2009 |
| Title |
Drosophila melanogaster61 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila L57 Cells JHIII 6h
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Drosophila L57 DMSO vs juvenile hormone III treated (3, 6 12h)
|
| Treatment protocol |
Cultured Drosophila L57 cells were exposed to 1 uM JH III for 3, 6 and 12 h.
|
| Growth protocol |
Drosophila L57 cells were grown and maintained at 26 ± 1°C in 25-cm2 cell culture flasks using HyQ CCM3 medium (HyClone, Logan, UT) with 5% FBS (heat inactivated, suitable for insect cells from Sigma).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from L57 cells (JH III-treated and DMSO-treated) was extracted with RNeasy Total RNA Isolation kit from QIAGEN (Valencia, CA) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS 3000 scanner.
|
| Description |
Gene expression data from DMSO treated and JH treated for 3, 6 and 12hr.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Feb 08, 2007 |
| Last update date |
Dec 29, 2008 |
| Contact name |
Subba Reddy Palli |
| Organization name |
University of Kentucky
|
| Department |
Entomology
|
| Street address |
S-225 Agr. Sci. Ctr. N
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40546 |
| Country |
USA |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE6999 |
A genome-wide survey for a Juvenile hormone response element, DR4, in the fruit fly, Drosophila melanogaster |
|
