|
| Status |
Public on Sep 04, 2015 |
| Title |
Liver_Control_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: female
strain: Sprague-Dawley
tissue: Liver
experimental group: control
age at death (days): 540
|
| Treatment protocol |
Livers were extracted at autopsy and half of the organ was snap frozen for RNA subsequent RNA isolation. Samples were stored at -80 until extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Frozen liver samples were homogenised and RNA extracted using MagMAX-96 for Micorarrays Kit (AM1839). The spin procedure was followed. RNA was eluted in 50ul of elution buffer.
|
| Label |
Biotin
|
| Label protocol |
RNA was processed using the Ambion WT Expression kit and Affymetrix WT Terminal Labelling kit.
|
| |
|
| Hybridization protocol |
Hybridisation cocktails were prepared using 5.5ug cDNA as described in the Eukaryotic Array manual from Affymetrix, using the Hybridisation, Wash and Stain (HWS) kit (Affymetrix)
|
| Scan protocol |
Arrays were scanned using the GCS3000 7G scanner (Affymetrix) following manufacturer's protocols.
|
| Data processing |
CEL files were imported into Expression Console software (Affymetrix) and normalised using RMA-sketch (gene-level).
|
| |
|
| Submission date |
Feb 18, 2015 |
| Last update date |
Sep 04, 2015 |
| Contact name |
Matthew Arno |
| E-mail(s) |
matthew.arno@kcl.ac.uk
|
| Phone |
442078484286
|
| Organization name |
King's College London
|
| Department |
Genomics Centre
|
| Lab |
Arno
|
| Street address |
150 Stamford Street
|
| City |
London |
| State/province |
LONDON |
| ZIP/Postal code |
SE1 9NH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17117 |
| Series (2) |
| GSE66058 |
Wide-scale transcriptome disturbance underlies liver and kidney pathology from chronic ultra low dose Roundup exposure [liver] |
| GSE66060 |
Wide-scale transcriptome disturbance underlies liver and kidney pathology from chronic ultra low dose Roundup exposure |
|
