|
| Status |
Public on May 05, 2016 |
| Title |
ES56: Zfp36l3 KO Placenta RNA-seq |
| Sample type |
SRA |
| |
|
| Source name |
placenta
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Zfp36l3 KO
development stage: Embryonic day 15.5
tissue type: Placenta
|
| Treatment protocol |
none
|
| Growth protocol |
Living mice; normal growth conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from embryonic day 15.5 placentas using the GE Healthcare RNA isolation kit. RNA was quantified using the Thermo Scientific Nanodrop 2000c.
Libraries were prepared according to Illumina's Instructions
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
raw reads were mapped to mm10 RefSeq cDNA sequences using BWA default paired-end read mapping protocol
the reads that were not mapped in-pair were filtered out
total read count of each transcript were reported and normalized by EpiCenter
FPKM data were derived from the normalized read counts
Genome_build: mm10
|
| |
|
| Submission date |
Feb 20, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Perry J Blackshear |
| E-mail(s) |
black009@niehs.nih.gov
|
| Phone |
919-541-4926
|
| Organization name |
NIEHS
|
| Street address |
101 Alexander Dr.
|
| City |
Durham |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL9250 |
| Series (2) |
| GSE66137 |
Gene expression differences in mouse placenta tissue bewteen wild-type and Zfp36l3 knockout mice |
| GSE66139 |
Deficiency of the placenta- and yolk sac-specific tristetraprolin family member ZFP36L3 identifies likely mRNA targets and an unexpected link to placental iron metabolism |
|
| Relations |
| BioSample |
SAMN03356920 |
| SRA |
SRX884222 |
