|
| Status |
Public on Feb 24, 2015 |
| Title |
FB032_LG+Prlo |
| Sample type |
SRA |
| |
|
| Source name |
mid-fetal cerebral cortex
|
| Organism |
Homo sapiens |
| Characteristics |
gestational age (weeks): 18
facs population: LeX+ Glast+ Prominin-low
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissue samples were dissociated with trypsin, immunolabeled, and FACS sorted, and RNA purified from sorted cell populations, all using standard kits and protocols.
Illumina TruSeq v2 paired-end mRNA library protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Outer radial glial cells, replicate 1
|
| Data processing |
base-calling with Illumina CASAVA 1.8.2
alignment to the human reference genome and UCSC Known Genes transcriptome with tophat v2.0.6
gene expression level estimation and differential expression analysis performed with cufflinks v2.0.2
Genome_build: hg19
Supplementary_files_format_and_content: cufflinks gene-level fpkm tracking file format, contains gene-level expression estimates for each condition summarized across the three biological replicates
|
| |
|
| Submission date |
Feb 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Matthew Bonser Johnson |
| E-mail(s) |
matthew.johnson@childrens.harvard.edu
|
| Organization name |
Boston Children's Hospital
|
| Department |
Genetics and Genomics
|
| Street address |
300 Longwood Ave.
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE66217 |
Single Cell Analysis Reveals Unexpected Transcriptional Heterogeneity of Neural Progenitors in the Developing Human Cortex |
|
| Relations |
| BioSample |
SAMN03366661 |
| SRA |
SRX886329 |
