NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1617815 Query DataSets for GSM1617815
Status Public on Mar 10, 2015
Title subject 13-007, proliferating
Sample type RNA
 
Source name subcutaneous adipocytes
Organism Homo sapiens
Characteristics subject: 13-007
cell type: abdominal subcutaneous preadipocyte
cell state: proliferating
Treatment protocol Preadipocytes were irradiated to induce senescence. Sham irradiated cells from the same subject were used as a control. RNA was isolated 20 days after induction of senecence.
Growth protocol Preadipocytes were grown in aMEM containing 10% FBS. In senescent cultures, medium was changed twice a week.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotin labeling was performed using the Ambion WT Expression Kit (Life Technologies, Grand Island, NY) according to the manufacturer's protocol, followed by the GeneChip WT Terminal Labeling and Controls Kit (Affymetrix, Santa Clara, CA).
 
Hybridization protocol The labeled, fragmented DNA was hybridized to the Affymetrix GeneChip Human Gene 1.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
Scan protocol After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus.
Description Sample name: 13-007_SQ
Gene expression data from subcutaneous adipocytes proliferating in culture.
Data processing Raw Affymetrix CEL files were normalized to produce Entrez Gene-identifier-specific expression values using the implementation of the Robust Multiarray Average (RMA) in the affy Bioconductor package (version 1.36.1), R (version 2.15.1), and the Brainarray hugene10sthsentrezg R package (version 16.0.0)
 
Submission date Feb 24, 2015
Last update date Jun 25, 2019
Contact name Boston University Microarray and Sequencing Resource
E-mail(s) msrdata@bu.edu
Organization name Boston University
Department Microarray and Sequencing Resource
Street address 72 East Concord Street, E631
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL17244
Series (1)
GSE66236 Achilles Heels of Senescent Cells: From Transcriptome to Senolytic Drugs

Data table header descriptions
ID_REF
VALUE log2-transformed, RMA-normalized Entrez Gene expression values

Data table
ID_REF VALUE
100009676_at 6.862891896
10000_at 9.316253904
10001_at 8.032900158
10002_at 5.295279484
100033413_at 5.576085966
100033414_at 5.699636519
100033416_at 6.446308489
100033418_at 7.015820862
100033420_at 8.415989783
100033422_at 2.546834651
100033423_at 3.891060013
100033424_at 3.091414818
100033425_at 3.693707381
100033426_at 10.05252913
100033427_at 11.63603583
100033428_at 1.984907549
100033430_at 2.070346862
100033431_at 7.677004156
100033432_at 5.747998743
100033433_at 3.851340073

Total number of rows: 19684

Table truncated, full table size 400 Kbytes.




Supplementary file Size Download File type/resource
GSM1617815_JK_B8_13-007_SQ.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap