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Sample GSM1619883 Query DataSets for GSM1619883
Status Public on Feb 25, 2018
Title CF968 IgG
Sample type RNA
 
Source name Peripheral blood mononuclear cells
Organism Homo sapiens
Characteristics cell type: Peripheral blood mononuclear cells
activation agent: AT1RaAB
time: 24hrs
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with RNeasy Mini Kit followed by DNase I treatment.
Label SYBR Green
Label protocol PCR assays were performed using the Dendritic Cell - Antigen Presenting Cell and the Toll-like Receptor Pathway PCR assay panel (SA Biosciences, Frederick, MD) following the Manufacturer’s instructions. Reverse transcription was performed from 500 ng total RNA using the RT2 First Strand Kit (SA Biosciences). Quantitative real-time PCR were performed (Light Cycler 480, Roche) with 45 cycles at 94 oC for 15 seconds and 60 oC for 60 seconds.
 
Hybridization protocol n/a
Scan protocol n/a
Description Test
Data processing The normalization and all the data analysis were performed according to the manufacturers instructions using their web-based software package: http://sabiosciences.com/pcrarraydataanalysis.php
For the normalization it uses the average of five housekeeping genes: B2M, HPRT1 , RPL13A, GAPDH, ACTB.
Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)]
The web-based software package automatically performs all deltadeltaCt based fold-change calculations from the uploaded raw threshold cycle data.
Fold Change worksheet reports test/control ratios.
 
Submission date Feb 26, 2015
Last update date Feb 25, 2018
Contact name Peter M Abadir
E-mail(s) PABADIR1@JHMI.EDU
Phone 4105505436
Organization name Johns Hopkins University
Department Geriatrics
Lab Abadir
Street address 5501 Hopkins Bayview Circle. Rm 1a62
City Baltimore
State/province Maryland
ZIP/Postal code 21224
Country USA
 
Platform ID GPL19826
Series (1)
GSE66331 Real-time quantitative PCR analysis of human monocyte cells treated invitro with purified angiotensin type 1 receptor auto antibody

Data table header descriptions
ID_REF
VALUE Matrix normalized worksheet reports normalized signal (against housekeeping genes). AVG Delta(Ct) (Ct(GOI) - Ave Ct(HKG))

Data table
ID_REF VALUE
A01 1.532035
A02 0.412035
A03 1.682035
A04 3.802035
A05 -0.067965
A06 -1.277965
A07 -1.117965
A08 1.332035
A09 0.712035
A10 -0.537965
A11 -2.977965
A12 -1.117965
B01 5.302035
B02 7.672035
B03 6.022035
B04 4.172035
B05 9.492035
B06 0.832035
B07 0.322035
B08 10.662035

Total number of rows: 96

Table truncated, full table size 1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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