|
| Status |
Public on May 10, 2007 |
| Title |
E2 slide 42 day 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
E2 - treatment hypothalamus, slide 42
|
| Organism |
Carassius auratus |
| Characteristics |
adult male goldfish, 2-3 years of age, exposed to silastic implant containing 17β-estradiol (E2) (100ug/g body mass), 1 day exposure
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent,
Quantarray scan
|
| Label |
Cy5
|
| Label protocol |
See Martyniuk et al. 2006 (PMID: 16954407) for details on microarray labelling.
|
| |
|
| Channel 2 |
| Source name |
E2 - control hypothalamus, slide 42
|
| Organism |
Carassius auratus |
| Characteristics |
adult male goldfish, 2-3 years of age, exposed to silastic implant, 1 day exposure
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent,
Quantarray scan
|
| Label |
Cy3
|
| Label protocol |
See Martyniuk et al. 2006 (PMID: 16954407) for details on microarray labelling.
|
| |
|
| |
| Hybridization protocol |
Genisphere protocol (http://www.genisphere.com/array_detection_home.html)
Cy5 Cy3 labelling
2ug RNA labelled
See Martyniuk et al. 2006 (PMID: 16954407) for details.
|
| Scan protocol |
10um scan
red 633nm and green 543nm laser.
|
| Description |
See Martyniuk et al. 2006 (PMID: 16954407) for details. Also please check the website about the goldfish microarraay project:
wwww.auratus.ca
|
| Data processing |
Lowess Normalization
|
| |
|
| Submission date |
Feb 13, 2007 |
| Last update date |
Feb 14, 2007 |
| Contact name |
Christopher Joseph Martyniuk |
| E-mail(s) |
CMARTYN@UFL.EDU
|
| Organization name |
UF
|
| Department |
Physiological Sciences
|
| Street address |
CEHT Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32611 |
| Country |
USA |
| |
|
| Platform ID |
GPL3735 |
| Series (1) |
| GSE7025 |
Auratus goldfish genomics project - E2 effect on male goldfish hypothalamus |
|
