|
| Status |
Public on Jul 01, 2007 |
| Title |
Mutant 2 with array type maize from Affymetrix |
| Sample type |
RNA |
| |
|
| Source name |
mutant2
|
| Organism |
Zea mays |
| Characteristics |
Zea mays (f252) mutant (+/bt2-H2328) - dev.stage (Boyes et al. Plant Cell 2001):35 days after pollinisation
|
| Treatment protocol |
no treatment
|
| Growth protocol |
seed - Grown in green house in Grodent substrate with 16 h day light plus artificial lighting and 8 h night with a temperature of 24degreeC/17degreeC. Humidity was not controlled but was approximately 70%/80%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mutant2:17ug.
|
| Label |
Biotin
|
| Label protocol |
labelling Biotin direct, amplification=yes, RNAa 20 ug.
|
| |
|
| Hybridization protocol |
labelled extract quantity: 15ug
|
| Scan protocol |
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
|
| Description |
The Brittle2 (Bt2) gene is one of three genes in the maize genome coding for the small subunit of ADP-glucose pyro-phosphorylase (AGPase), a key enzyme in starch synthesis. It is predominantly expressed in the developing endosperm. The bt2 mutant is characterised by collapsed, brittle kernels on mature ears. During kernel development a strong increase in soluble sugars (mainly sucrose) and a strong reduction in starch content has been observed. The goal of the present study was to compare the transcriptome between wildtype and bt2 kernels (bt2-H2328 allele) at mid-development (35 days after pollination). The main biological question was, whether the block in starch synthesis caused the transcriptional induction of other metabolic pathways to allow an alternative use of the excess sucrose.
|
| Data processing |
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
|
| |
|
| Submission date |
Feb 14, 2007 |
| Last update date |
Feb 21, 2007 |
| Contact name |
sandrine balzergue |
| Organization name |
INRA
|
| Lab |
IRHS
|
| Street address |
Rue G. Morel
|
| City |
Beaucouze |
| ZIP/Postal code |
49000 |
| Country |
France |
| |
|
| Platform ID |
GPL4032 |
| Series (1) |
| GSE7030 |
Phenotypic and molecular characterisation of a novel Bt2 allele in maize |
|
