|
| Status |
Public on Mar 05, 2015 |
| Title |
knee articular cartilage at T1, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Rattus norvegicus knee articular cartilage in adult
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
tissue: knee articular cartilage
developmental stage: youth
|
| Treatment protocol |
After grinded under the ice, cartilage tissue were collected for further total RNA extraction.
|
| Growth protocol |
The cartilage that was harvested individually from the right knee joint were stored at liquid nitrogen temperatures at once with protected by Rnasin inhibitor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
|
| Description |
Rattus norvegicus_T1-3
L43_(RaGene-2_0-st).rma-gene-full-Signal
Gene expression data from the rat knee articular cartilage in youth
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 05, 2015 |
| Last update date |
Mar 05, 2015 |
| Contact name |
Xiao Chun Wei |
| E-mail(s) |
weixc666@163.com
|
| Phone |
86-0351-3365579
|
| Organization name |
the Second Hospital of Shanxi Medical University
|
| Department |
Department of Orthopaedics
|
| Lab |
Shanxi Key Laboratory of Bone and Soft Tissue Injury Repair
|
| Street address |
No.382, Wuyi Road,Taiyuan City.
|
| City |
Taiyuan City |
| State/province |
Shanxi Shanxi |
| ZIP/Postal code |
030001 |
| Country |
China |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE66554 |
Expression data of LncRNA from the rat (Sprague-Dawley) knee articular cartilage at different developmental stages |
|
