|
| Status |
Public on Mar 07, 2015 |
| Title |
germline stem cell line 2, replicate a |
| Sample type |
RNA |
| |
|
| Source name |
germline stem cell line
|
| Organism |
Mus musculus |
| Characteristics |
strain background: OG2
cell type: germline stem cells (GSCs)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNeasy Mini Kit (Qiagen)
|
| Label |
biotin/streptavidin-Cy3
|
| Label protocol |
Biotinylated cRNA was prepared using the TotalPrep RNA Amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
GSC#2_a
|
| Data processing |
Raw data was processed by mapping bead intensities using BeadStudio 3.2 (Illumina), performing background correction using the Robust Multiarray Analysis (Affymetrix), normalizing gene expression using the lumi package of R‑Bioconductor, and performing variance stabilization by log2 scaling.
|
| |
|
| Submission date |
Mar 06, 2015 |
| Last update date |
Mar 07, 2015 |
| Contact name |
Ulf Tiemann |
| E-mail(s) |
ulf.tiemann@gmail.com
|
| Organization name |
Heinrich Heine University Duesseldorf
|
| Department |
Institute for Stem Cell Research and Regenerative Medicine
|
| Lab |
Natalia Tapia Lab
|
| Street address |
Moorenstrasse 5
|
| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE66613 |
Epigenetic Memory Is Not Intrinsic To Transcription Factor-Mediated Reprogramming [Illumina array] |
| GSE67026 |
Epigenetic Memory Is Not Intrinsic To Transcription Factor-Mediated Reprogramming |
|
