|
| Status |
Public on May 01, 2015 |
| Title |
CP70-DDB2-2 |
| Sample type |
RNA |
| |
|
| Source name |
CP70 cells transfected with DDB2-expressing vector, clone-2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: ovarian cancer cell line A2780-CP70
protocol: DDB2-overexpression
|
| Growth protocol |
Cells were grown in RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), and maintained in a humidified atmosphere with 5% CO2 at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Amplified and biotinylated sense-strand DNA were prepared according to the standard Affymetrix protocol from 100ng total RNA
|
| |
|
| Hybridization protocol |
Following fragment and label, 5.2 g of single-strand DNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human transcriptome array 2.0. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from CP70 cells transfected with DDB2-expressing vector
|
| Data processing |
affymetrix-algorithm-name = rma-gene-full
affymetrix-algorithm-version = 1.0
|
| |
|
| Submission date |
Mar 06, 2015 |
| Last update date |
May 01, 2015 |
| Contact name |
Qi-En Wang |
| E-mail(s) |
qi-en.wang@osumc.edu
|
| Phone |
614-292-9012
|
| Organization name |
The Ohio State University
|
| Department |
Radiation Oncology
|
| Street address |
420 W.12th Avenue
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE66636 |
DDB2 regulates gene expression in ovarian cancer cell lines |
|
