|
| Status |
Public on Apr 01, 2015 |
| Title |
Sample7 |
| Sample type |
genomic |
| |
|
| Source name |
AML
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: acute myeloid leukemia (AML)
|
| Growth protocol |
Standard protocol
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from mononuclear cell preparations of leukemic blasts obtained from peripheral blood or bone marrow at diagnosis using the Qiagen DNA Blood Kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, Qubit and 2% agarose gel electrophoresis.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
not provided
|
| Description |
Hybridized to 250K_Nsp
|
| Data processing |
The array image was acquired using Affymetrix GeneChip® Operating Software (GCOS version 1.4). Copy number values for individual SNPs were extracted and converted from CEL files into signal intensities using Affymetrix Copy Number Analysis Tool (CNAT 4.0.1) and Affymetrix Genotyping Console Software (GCOS 4.0.0).
|
| |
|
| Submission date |
Mar 09, 2015 |
| Last update date |
Apr 01, 2015 |
| Contact name |
Bartlomiej Przychodzen |
| E-mail(s) |
przychb@ccf.org
|
| Organization name |
Cleveland Clinic
|
| Department |
THOR
|
| Street address |
9600 Euclid Avenue
|
| City |
Cleveland |
| State/province |
Ohio |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platform ID |
GPL3718 |
| Series (1) |
| GSE66668 |
Inherited and somatic defects in DDX41 in myeloid neoplasms |
|
