|
| Status |
Public on Mar 11, 2015 |
| Title |
reproductively inactive chicken rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Pooled pituitary glands of 5 reproductively inactive chickens
|
| Organism |
Gallus gallus |
| Characteristics |
gender: Female
developmental stage: Pullet
reproductive stage: non-laying
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen) following manufacturer’s instructions
|
| Label |
Alexa fluor 555, Alexa fluor 647
|
| Label protocol |
cDNA was synthesized and fluorescently labelled using SuperScript Plus Indirect cDNA Labeling System (Invitrogen) following manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Hybridisation was performed at 42°C for 16 hr using SlideHyb Buffer (Applied Biosystems) in hybridization chambers. The arrays were washed with increasing stringency using salt sodium citrate and immediately scanned
|
| Scan protocol |
The arrays were scanned on a ScanArray 5000 (Packard BioScience)
|
| Data processing |
The fluorescence intensity of each spot from the images was extracted using BlueFuse software (BlueGnome Ltd.) and exported to JMP Genomics (SAS Institute Inc.). The raw intensity was first transformed to log-base 2 and performed within-array Loess normalization and then standardized to a mean of 0 and standard deviation of 1 across arrays.
|
| |
|
| Submission date |
Mar 09, 2015 |
| Last update date |
Mar 11, 2015 |
| Contact name |
Sunantha Kosonsiriluk |
| E-mail(s) |
koson003@umn.edu
|
| Organization name |
University of Minnesota
|
| Department |
Animal Science
|
| Lab |
El Halawani
|
| Street address |
495 AnSci/VetMed 1988 Fitch Ave
|
| City |
Saint Paul |
| State/province |
Minnesota |
| ZIP/Postal code |
55108 |
| Country |
USA |
| |
|
| Platform ID |
GPL19870 |
| Series (1) |
| GSE66704 |
Validation of chicken array for gene expression analysis in turkey |
|
